Mimicking Insect Communication: Release and Detection of Pheromone, Biosynthesized by an Alcohol Acetyl Transferase Immobilized in a Microreactor

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Muñoz, Lourdes ; Dimov, Nikolay ; Carot-Sans, Gerard ; Bula, Wojciech P. ; Guerrero, Angel ; J. G. E. Gardeniers, Han (2012)
  • Publisher: Figshare
  • Related identifiers: doi: 10.1371/journal.pone.0047751
  • Subject: Biochemistry | Molecular Biology | Neuroscience | Biotechnology | Chemistry | mimicking | detection | biosynthesized | acetyl | transferase | immobilized | microreactor

<div><p>Infochemical production, release and detection of <em>(Z,E)</em>-9,11-tetradecadienyl acetate, the major component of the pheromone of the moth <em>Spodoptera littoralis</em>, is achieved in a novel microfluidic system designed to mimic the final step of the pheromone biosynthesis by immobilized recombinant alcohol acetyl transferase. The microfluidic system is part of an “artificial gland”, i.e., a chemoemitter that comprises a microreactor connected to a microevaporator and is able to produce and release a pre-defined amount of the major component of the pheromone from the corresponding <em>(Z,E)</em>-9,11-tetradecadienol. Performance of the entire chemoemitter has been assessed in electrophysiological and behavioral experiments. Electroantennographic depolarizations of the pheromone produced by the chemoemitter were ca. 40% relative to that evoked by the synthetic pheromone. In a wind tunnel, the pheromone released from the evaporator elicited on males a similar attraction behavior as 3 virgin females in most of the parameters considered.</p> </div>
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