IncA/C Plasmid-Mediated Spread of CMY-2 in Multidrug-Resistant Escherichia coli from Food Animals in China

Collection UNKNOWN
Guo, Yu-Fang ; Zhang, Wen-Hui ; Ren, Si-Qi ; Yang, Lin ; Lü, Dian-Hong ; Zeng, Zhen-Ling ; Liu, Ya-Hong ; Jiang, Hong-Xia (2014)
  • Publisher: Figshare
  • Related identifiers: doi: 10.1371/journal.pone.0096738
  • Subject: microbial pathogens | escherichia coli | organismal evolution | veterinary science | infectious diseases | emerging infectious diseases | microbiology | microbial evolution | bacterial pathogens | Biological Sciences | multidrug-resistant | medical microbiology | bacteriology | cmy-2 | evolutionary biology | veterinary microbiology | china | bacterial evolution | animals | plasmid-mediated
    mesheuropmc: bacterial infections and mycoses | biochemical phenomena, metabolism, and nutrition

<div><p>Objectives</p><p>To obtain a broad molecular epidemiological characterization of plasmid-mediated AmpC β-lactamase CMY-2 in <i>Escherichia coli</i> isolates from food animals in China.</p><p>Methods</p><p>A total of 1083 <i>E. coli</i> isolates from feces, viscera, blood, drinking water, and sub-surface soil were examined for the presence of CMY-2 β-lactamases. CMY-2-producing isolates were characterized as follows: the <i>bla</i><sub>CMY-2</sub> genotype was determined using PCR and sequencing, characterization of the <i>bla</i><sub>CMY-2</sub> genetic environment, plasmid sizing using S1 nuclease pulsed-field gel electrophoresis (PFGE), PCR-based replicon typing, phylogenetic grouping, <i>Xba</i>I-PFGE, and multi-locus sequence typing (MLST).</p><p>Results</p><p>All 31 CMY-2 producers were only detected in feces, and presented with multidrug resistant phenotypes. All CMY-2 strains also co-harbored genes conferring resistance to other antimicrobials, including extended spectrum β-lactamases genes (<i>bla</i><sub>CTX-M-14</sub> or <i>bla</i><sub>CTX-M-55</sub>), plasmid-mediated quinolone resistance determinants (<i>qnr</i>, <i>oqxA</i>, and <i>aac-(6′)-Ib-cr</i>), <i>floR</i> and <i>rmtB</i>. The co-transferring of <i>bla</i><sub>CMY-2</sub> with <i>qnrS1</i> and <i>floR</i> (alone and together) was mainly driven by the Inc A/C type plasmid, with sizes of 160 or 200 kb. Gene cassette arrays inserted in the class 1 or class 2 integron were amplified among 12 CMY-2 producers. CMY-2 producers belonged to avirulent groups B1 (<i>n</i> = 12) and A (<i>n</i> = 11), and virulent group D (<i>n</i> = 8). There was a good correlation between phylogenetic groups and sequence types (ST). Twenty-four STs were identified, of which the ST complexes (STC) 101/B1 (<i>n</i> = 6), STC10/A (<i>n</i> = 5), and STC155/B1 (<i>n</i> = 3) were dominant.</p><p>Conclusions</p><p>CMY-2 is the dominant AmpC β-lactamase in food animals and is associated with a transferable replicon IncA/C plasmid in the STC101, STC10, and STC155 strains.</p></div>
Share - Bookmark

  • Download from
    figshare via figshare (Collection, 2014)
  • Cite this research product