Fluorescent Protein Based FRET Pairs with Improved Dynamic Range for Fluorescence Lifetime Measurements
George Abraham, Bobin
S. Sarkisyan, Karen
S. Mishin, Alexander
Tkachenko, Nikolai V.
- Publisher: Figshare
fluorescence lifetime measurements | F örster radius | Fluorescence Lifetime Measurements Fluorescence Resonance Energy Transfer | flim | fluorescence lifetime imaging microscopy | FRET pairs | Protein Variants | FRET efficiency
mesheuropmc: biological sciences
<div><p>Fluorescence Resonance Energy Transfer (FRET) using fluorescent protein variants is widely used to study biochemical processes in living cells. FRET detection by fluorescence lifetime measurements is the most direct and robust method to measure FRET. The traditi...