Controlled Growth and the Maintenance of Human Pluripotent Stem Cells by Cultivation with Defined Medium on Extracellular Matrix-Coated Micropatterned Dishes

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Takenaka, Chiemi; Miyajima, Hiroshi; Yoda, Yusuke; Imazato, Hideo; Yamamoto, Takako; Gomi, Shinichi; Ohshima, Yasuhiro; Kagawa, Kenichi; Sasaki, Tetsuji; Kawamata, Shin;
  • Publisher: Figshare
  • Related identifiers: doi: 10.1371/journal.pone.0129855
  • Subject: Uncategorised | spm | hpsc | Human Pluripotent Stem Cells | sequential adaptation process | process quality control | tra | cell culture system | culture system | Comparative flow cytometric studies | quality control tests | ssea

<div><p>Here, we introduce a new serum-free defined medium (SPM) that supports the cultivation of human pluripotent stem cells (hPSCs) on recombinant human vitronectin-N (rhVNT-N)-coated dishes after seeding with either cell clumps or single cells. With this system, the... View more
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