research product . 2019

Use of the pre-assembled ribonucleoprotein (RNP) complex for gene editing of wheat and poplar

Sparks, C. A.; Cerezo-Medina, S.; Raffan, S.; Hahn, F.;
Open Access
  • Published: 01 Jan 2019
  • Country: United Kingdom
Abstract
Genetic modification has been a useful research tool to study overexpression and down-regulation of genes. Gene editing offers a much more targeted approach to make more subtle changes to genes already present within the plant. However, integration of Cas9 and guide RNA sequences into the genome can be disadvantageous causing offsite cleavage and/or continued cleavage of target sites in subsequent generations. Use of purified protein could overcome this issue and would also avoid reliance on the cell’s expression machinery thus making effective promoters less essential. The use of purified proteins would also address regulatory issues as no recombinant DNA of an...
Subjects
Medical Subject Headings: food and beverages
free text keywords: Gene editing, Ribonucleoprotein complex, Wheat, Poplar, Biolistics
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