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Other research product . Other ORP type . 2022

DNA sequence baits targeting ultra-conserved elements in marine invertebrates

Banta, Gary T.; Winding Hansen, Benni.; Knott, Emily; Petersen, Haidi;
Open Access
English
Published: 12 Apr 2022
Country: Finland
Abstract

This dataset includes four files of DNA sequence baits targeted to ultra-conserved elements (UCEs) identified from four different taxonomic groups of marine invertebrates: Annelida, Bivalvia, Gastropoda and Crustacea. UCE loci are genomic regions of high conservation that can be found throughout the genomes that can be extracted from the genome and sequenced for use in phylogenetic, phylogeographic and population genetic analyses. The UCE loci were located by aligning a minimum of five species’ genomes to a base genome in each taxonomic group and searching for overlapping highly conserved areas. The UCE baits were designed with screening coverage of 4X using the pipeline phyluce v. 1.6.6 (https://github.com/faircloth-lab/phyluce; Faircloth 2015). The analysis generated 3316 baits for 590 conserved loci in annelids, 4110 baits for 519 loci in bivalves, 25547 baits for 4367 loci in gastropods and 27545 baits for 3755 conserved loci in crustaceans. A final set of 3000 baits per taxonomic group (12000 baits in total) was created by Arbor Biosciences (Ann Arbor, MI, USA, arborbiosci.com) using their myBaits probe design support. For more detailed documentation, see the downloadable dataset description file.

Subjects

genome, hybridisation, animal populations, risteytyminen, perimä, eläinkannat, DNA

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