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Vilnius University

Country: Lithuania

Vilnius University

115 Projects, page 1 of 23
  • Funder: EC Project Code: 293476
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  • Funder: EC Project Code: 101087964
    Overall Budget: 1,999,490 EURFunder Contribution: 1,999,490 EUR

    Crop diversification critically mitigates agricultural risk for humanity. Identifying the environmental and cultural factors influencing the adoption and abandonment of staple foods through time is essential to an informed discussion about present-day food security and adaptation to a changing global climate. Past studies on ancient agriculture have identified the routes and timing of primary crop dispersals, but we possess a remarkably narrow understanding on how and why new foods were integrated and later abandoned by societies, and why certain crops remained restricted to distinct geographical regions. The MILWAYS project is perfectly poised to fill this alarming gap in knowledge, though a multi-faceted investigation of a specific crop - broomcorn millet (Panicum miliaceum) - which, due to its unique biochemical properties, is traceable across space and time. Making use of multi-disciplinary cutting edge research methodologies, MILWAYS will bridge a large geographic territory and track the earliest millet dispersals across eastern-central Europe from the mid. 2nd mill. BCE onwards all the way to past millet cultivation limit. MILWAYS will utilize the high carbon isotope values of millet, resulting from its C4-photosynthetic pathway in conjunction with the distinct miliacin biomarker in order to identify its consumption, with respect to shifting climates, human mobility, demographic categories of sex, age and changes of plant use across historical times. Along with transforming the approaches on how we study past agriculture, MILWAYS will: a) Identify the interplay of cultural versus climatic factors in past staple food adoption and abandonment; b) Develop novel methodologies to the study of past diets and climates that are highly transferable to the study of other crops; c) Better understand millet environmental adaptation in northern latitudes; d) Create models of past and future crop exploitation strategies.

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  • Funder: EC Project Code: 620745
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  • Funder: EC Project Code: 300121
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  • Funder: EC Project Code: 705791
    Overall Budget: 130,780 EURFunder Contribution: 130,780 EUR

    Tackling heterogeneous cell populations at single-cell resolution is becoming increasingly important in different branches of biology and biomedicine. Many useful techniques have been developed to profile and even selectively purify single-cells, however, the demand for techniques with better analytical performance and improved high-throughput capabilities, remains very high. Droplet microfluidics can fulfill this demand by bringing higher throughput, scalability and single molecule resolution that are hard to achieve with conventional technologies. In this project, a droplet microfluidics platform will be developed and applied for ultra-high-throughput single-cell screening and sequencing. The project will be focused on B-cells that produce therapeutic antibodies or biomolecules of industrial interest. Cell compartmentalization into microfluidic droplets together with capture beads and barcoded DNA primers will enable a direct establishment of the linkage between the genotype (genes or mRNA) and phenotype (binding, regulatory or activity of secreted proteins). The proposed work will allow the quantitative high-throughput antibody phenotyping without loosing the original heavy-light chain pairing, a significant advantage over other technologies. Like no other system available to-date this the technological approach outlined in this proposal will provide a unique way to identify the primary sequence of heavy and light IgG genes encoding functional monoclonal antibodies directly from single-cells, without a need to perform gene cloning or cell immortalization. The results of this work are likely to bring a significant impact not only in applied biological sciences but also in biotechnology and biomedicine.

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