1 Research products, page 1 of 1

  • Open Access
    Charlotte Balière; Alain Rincé; Jorge Blanco; Ghizlane Dahbi; Josée Harel; Philippe Vogeleer; Jean-Christophe Giard; Patricia Mariani-Kurkdjian; Michèle Gourmelon;
    Publisher: Frontiers Media
    Countries: Spain, France, France, France, France

    During a 2-years study, the presence of Shiga-toxin producing Escherichia coli (STEC) and enteropathogenic E. coli (EPEC) was investigated in shellfish (n = 238), seawater (n = 12), and surface sediment (n = 39) collected from three French coastal shellfishharvesting areas and freshwaters (n=216) in their watersheds. PCR detection of Shiga toxin- (stx1/stx2) and intimin- (eae) genes following enrichment from these samples revealed the presence of least one of the stx genes in 30.3% of shellfish batches, 85.9% of freshwater, 41.7% of seawater, and 28.2% of sediment samples, while the eae gene was observed in 74.8, 100, 100, and 43.6% of shellfish batches, freshwater, seawater, and sediment samples, respectively. Twenty-eight STEC and 89 EPEC strains were isolated and analyzed in order to determine their serotype, phylogroup, and genetic relatedness and to evaluate the presence of the saa and ehxA genes encoding the STEC autoagglutinating adhesin and the enterohemolysin A, respectively. Finally, the ability to form biofilms and antimicrobial susceptibility were investigated for a selection of strains. Eighteen serotypes were identified among the STEC isolates and 57 among the EPEC isolates. A high diversity was observed within these strains, as 79 different PFGE patterns and 48 distinguishable sequence types were identified. Strains were found to belong mainly to phylogroups B1 and B2 and virulence was observed to be low as more than 85% of the strains possessed only stx1, stx2, or eae genes. One STEC and several EPEC strains belonged to three of the five highly pathogenic serogroups (i.e., O26, O103, and O145). The subset of strains tested for their capacity to form biofilms was mainly strongly to moderately adherent and more strains formed a strong biofilm at 18 than at 30◦C. Finally, more than 85% of analyzed strains were found to be sensitive to the 16 tested antibiotics. These data suggest the low risk of human infection by STEC if shellfish from these shellfishharvesting areas were consumed This work was funded by the European Regional Development Fund Interreg IVA Programme, as part of the collaborative project RiskManche. The thesis of CB was supported by a grant from Ifremer and the Agence de l’Eau Loire-Bretagne. We thank Clémence Balière from the University of Caen and Céline Courroux from Hôpital Robert-Debré, for their participation in the sampling and technical assistance and Dominique HervioHeath (Ifremer) and Huw Taylor (University of Brighton, UK) for their critical review of the manuscript. Work in the LRECUSC-laboratory was financed by the grant CN2012/303 from Consellería de Cultura, Educación e Ordenación Universitaria (Xunta de Galicia) and The European Regional Development Fund,ERDF) SI

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