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  • Open Access English
    Authors: 
    Vincenzo Torraca; Myrsini Kaforou; Jayne Watson; Gina Duggan; Hazel Guerrero-Gutierrez; Sina Krokowski; Michael Hollinshead; Thomas B. Clarke; Rafal Mostowy; Gillian S. Tomlinson; +3 more
    Publisher: Public Library of Science (PLoS)
    Countries: Poland, United Kingdom
    Project: WT , EC | MYCO TRAPS (700088), WT | Controlling the intracell... (097411), WT | Use of the cytoskeleton t... (206444), EC | ENTRAPMENT (772853)

    Shigella flexneri is historically regarded as the primary agent of bacillary dysentery, yet the closely-related Shigella sonnei is replacing S. flexneri, especially in developing countries. The underlying reasons for this dramatic shift are mostly unknown. Using a zebrafish (Danio rerio) model of Shigella infection, we discover that S. sonnei is more virulent than S. flexneri in vivo. Whole animal dual-RNAseq and testing of bacterial mutants suggest that S. sonnei virulence depends on its O-antigen oligosaccharide (which is unique among Shigella species). We show in vivo using zebrafish and ex vivo using human neutrophils that S. sonnei O-antigen can mediate neutrophil tolerance. Consistent with this, we demonstrate that O-antigen enables S. sonnei to resist phagolysosome acidification and promotes neutrophil cell death. Chemical inhibition or promotion of phagolysosome maturation respectively decreases and increases neutrophil control of S. sonnei and zebrafish survival. Strikingly, larvae primed with a sublethal dose of S. sonnei are protected against a secondary lethal dose of S. sonnei in an O-antigen-dependent manner, indicating that exposure to O-antigen can train the innate immune system against S. sonnei. Collectively, these findings reveal O-antigen as an important therapeutic target against bacillary dysentery, and may explain the rapidly increasing S. sonnei burden in developing countries. Funder: Lister Institute of Preventive Medicine; funder-id: http://dx.doi.org/10.13039/501100001255

Include:
1 Research products, page 1 of 1
  • Open Access English
    Authors: 
    Vincenzo Torraca; Myrsini Kaforou; Jayne Watson; Gina Duggan; Hazel Guerrero-Gutierrez; Sina Krokowski; Michael Hollinshead; Thomas B. Clarke; Rafal Mostowy; Gillian S. Tomlinson; +3 more
    Publisher: Public Library of Science (PLoS)
    Countries: Poland, United Kingdom
    Project: WT , EC | MYCO TRAPS (700088), WT | Controlling the intracell... (097411), WT | Use of the cytoskeleton t... (206444), EC | ENTRAPMENT (772853)

    Shigella flexneri is historically regarded as the primary agent of bacillary dysentery, yet the closely-related Shigella sonnei is replacing S. flexneri, especially in developing countries. The underlying reasons for this dramatic shift are mostly unknown. Using a zebrafish (Danio rerio) model of Shigella infection, we discover that S. sonnei is more virulent than S. flexneri in vivo. Whole animal dual-RNAseq and testing of bacterial mutants suggest that S. sonnei virulence depends on its O-antigen oligosaccharide (which is unique among Shigella species). We show in vivo using zebrafish and ex vivo using human neutrophils that S. sonnei O-antigen can mediate neutrophil tolerance. Consistent with this, we demonstrate that O-antigen enables S. sonnei to resist phagolysosome acidification and promotes neutrophil cell death. Chemical inhibition or promotion of phagolysosome maturation respectively decreases and increases neutrophil control of S. sonnei and zebrafish survival. Strikingly, larvae primed with a sublethal dose of S. sonnei are protected against a secondary lethal dose of S. sonnei in an O-antigen-dependent manner, indicating that exposure to O-antigen can train the innate immune system against S. sonnei. Collectively, these findings reveal O-antigen as an important therapeutic target against bacillary dysentery, and may explain the rapidly increasing S. sonnei burden in developing countries. Funder: Lister Institute of Preventive Medicine; funder-id: http://dx.doi.org/10.13039/501100001255

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