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  • Open Access
    Authors: 
    Zhixiong Hu; Andrew Glidle; Charles N. Ironside; Jonathan M. Cooper; Huabing Yin;
    Publisher: Royal Society of Chemistry (RSC)
    Country: United Kingdom
    Project: EC | BIO-PHONONICS (340117), UKRI | Engineering a semi-biotic... (EP/H04986X/1)

    We describe the development of an integrated lensed Arrayed Waveguide Grating (AWG) microspectrometer for localized multiplexing fluorescence measurements. The device, which has a footprint that is only 1 mm wide and 1 cm long, is capable of spectroscopic measurements on chip. Multiple fluorescence signals were measured simultaneously based upon simple intensity readouts from a CCD camera. We also demonstrate the integration of the AWG spectrometer with a microfluidic platform using a lensing function to confine the beam shape for focused illumination. This capability enhances signal collection, gives better spatial resolution, and provides a route for the analysis of small volume samples (e.g. cells) in flow. To show these capabilities we developed a novel “bead–AWG” platform with which we demonstrate localized multiplexed fluorescence detection either simultaneously or successively. Such an integrated system provides the basis for a portable system capable of optical detection of multi-wavelength fluorescence from a single defined location.

Include:
1 Research products, page 1 of 1
  • Open Access
    Authors: 
    Zhixiong Hu; Andrew Glidle; Charles N. Ironside; Jonathan M. Cooper; Huabing Yin;
    Publisher: Royal Society of Chemistry (RSC)
    Country: United Kingdom
    Project: EC | BIO-PHONONICS (340117), UKRI | Engineering a semi-biotic... (EP/H04986X/1)

    We describe the development of an integrated lensed Arrayed Waveguide Grating (AWG) microspectrometer for localized multiplexing fluorescence measurements. The device, which has a footprint that is only 1 mm wide and 1 cm long, is capable of spectroscopic measurements on chip. Multiple fluorescence signals were measured simultaneously based upon simple intensity readouts from a CCD camera. We also demonstrate the integration of the AWG spectrometer with a microfluidic platform using a lensing function to confine the beam shape for focused illumination. This capability enhances signal collection, gives better spatial resolution, and provides a route for the analysis of small volume samples (e.g. cells) in flow. To show these capabilities we developed a novel “bead–AWG” platform with which we demonstrate localized multiplexed fluorescence detection either simultaneously or successively. Such an integrated system provides the basis for a portable system capable of optical detection of multi-wavelength fluorescence from a single defined location.

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