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  • Open Access English
    Authors: 
    Christian Hazael Pérez-Beltrán; Juan José García-Guzmán; Bibiana I. Ferreira; O. Estévez-Hernández; David López-Iglesias; Laura Cubillana-Aguilera; Wolfgang Link; N. Stănică; Ana M. Rosa da Costa; José María Palacios-Santander;
    Publisher: Elsevier
    Countries: Portugal, Spain
    Project: EC | TRIBBLES (748585), FCT | UID/BIM/04773/2013 (UID/BIM/04773/2013)

    Christian Hazael Pérez-Beltrán acknowledges Universidad Autónoma de Sinaloa (México) for funding and supporting with a scholarship his studies of the Erasmus Mundus Master in Quality in Analytical Laboratories (EMQAL) and the EMQAL consortium for the opportunity of performing this work. The Spanish authors greatly acknowledge Junta de Andalucía and Institute of Research on Electron Microscopy and Materials (IMEYMAT – BIOSENSEP and APPLIED-SENS projects) for their financial support and ‘Programa de fomento e impulso de la investigación y de la transferencia de la Universidad de Cádiz 2018-2019’ for the funds given through the projects PR2018-070 (Proyectos de Investigación-Puente 2018) and PR2020-013/SMAGAC (Proyectos de Investigación-Puente 2020). Dr. Osvaldo L. Estévez-Hernández thanks ‘Programa de Movilidad Postdoctoral’ funded by Asociación Universitaria Iberoamericana de Posgrado (AUIP) for his postdoc fellowship. This work was also supported by Fundação para a Ciência e a Tecnologia (FCT) Research Center Grant UID/BIM/04773/2013 Centre for Biomedical Research (CBMR), and UID/QUI/04023/2019 Algarve Chemistry Research Centre (CIQA), as well as by the Spanish Ministry of Science, Innovation and Universities through Grant RTI2018-094629-B-I00 to WL. BIF was supported by FCT-SFRH/BPD/100434/2014 and Marie Curie Individual Fellowship project TRIBBLES (#748585). This work was also supported by two LPCC-NRS/Terry Fox grants (2016/2017; 2017/2018). The present study is focused on the ultrafast and green synthesis, via the co-precipitation method, of magnetic nanoparticles (MNPs) based on iron oxides using design of experiments (DOE) and high energy sonochemical approach, considering two main factors: amplitude (energy) of the ultrasound probe and sonication time. The combination of these techniques allowed the development of a novel one-minute green synthesis, which drastically reduced the amount of consumed energy, solvents, reagents, time and produced residues. This green sonochemical synthesis permitted to obtain mean particle sizes of 11 ± 2 nm under the optimized conditions of amplitude = 40% (2826 J) and time = 1 min. Their composition, structure, size, morphology and magnetic properties were assessed through X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), thermogravimetric analysis (TGA), scanning and transmission electron microscopy (SEM & TEM), and vibrating sample magnetometry (VSM). The characterization results indicate the proper formation of MNPs, and the correct functionalization of MNPs with different coating agents. The functionalized MNPs were used as: i) biosensor, which could detect mercury in water in the range of 0.030–0.060 ppm, and ii) support onto which polyclonal antibodies were anchored and successfully bound to an osteosarcoma cell line expressing the target protein (TRIB2-GFP), as part of an immunoprecipitation assay.

Include:
1 Research products, page 1 of 1
  • Open Access English
    Authors: 
    Christian Hazael Pérez-Beltrán; Juan José García-Guzmán; Bibiana I. Ferreira; O. Estévez-Hernández; David López-Iglesias; Laura Cubillana-Aguilera; Wolfgang Link; N. Stănică; Ana M. Rosa da Costa; José María Palacios-Santander;
    Publisher: Elsevier
    Countries: Portugal, Spain
    Project: EC | TRIBBLES (748585), FCT | UID/BIM/04773/2013 (UID/BIM/04773/2013)

    Christian Hazael Pérez-Beltrán acknowledges Universidad Autónoma de Sinaloa (México) for funding and supporting with a scholarship his studies of the Erasmus Mundus Master in Quality in Analytical Laboratories (EMQAL) and the EMQAL consortium for the opportunity of performing this work. The Spanish authors greatly acknowledge Junta de Andalucía and Institute of Research on Electron Microscopy and Materials (IMEYMAT – BIOSENSEP and APPLIED-SENS projects) for their financial support and ‘Programa de fomento e impulso de la investigación y de la transferencia de la Universidad de Cádiz 2018-2019’ for the funds given through the projects PR2018-070 (Proyectos de Investigación-Puente 2018) and PR2020-013/SMAGAC (Proyectos de Investigación-Puente 2020). Dr. Osvaldo L. Estévez-Hernández thanks ‘Programa de Movilidad Postdoctoral’ funded by Asociación Universitaria Iberoamericana de Posgrado (AUIP) for his postdoc fellowship. This work was also supported by Fundação para a Ciência e a Tecnologia (FCT) Research Center Grant UID/BIM/04773/2013 Centre for Biomedical Research (CBMR), and UID/QUI/04023/2019 Algarve Chemistry Research Centre (CIQA), as well as by the Spanish Ministry of Science, Innovation and Universities through Grant RTI2018-094629-B-I00 to WL. BIF was supported by FCT-SFRH/BPD/100434/2014 and Marie Curie Individual Fellowship project TRIBBLES (#748585). This work was also supported by two LPCC-NRS/Terry Fox grants (2016/2017; 2017/2018). The present study is focused on the ultrafast and green synthesis, via the co-precipitation method, of magnetic nanoparticles (MNPs) based on iron oxides using design of experiments (DOE) and high energy sonochemical approach, considering two main factors: amplitude (energy) of the ultrasound probe and sonication time. The combination of these techniques allowed the development of a novel one-minute green synthesis, which drastically reduced the amount of consumed energy, solvents, reagents, time and produced residues. This green sonochemical synthesis permitted to obtain mean particle sizes of 11 ± 2 nm under the optimized conditions of amplitude = 40% (2826 J) and time = 1 min. Their composition, structure, size, morphology and magnetic properties were assessed through X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), thermogravimetric analysis (TGA), scanning and transmission electron microscopy (SEM & TEM), and vibrating sample magnetometry (VSM). The characterization results indicate the proper formation of MNPs, and the correct functionalization of MNPs with different coating agents. The functionalized MNPs were used as: i) biosensor, which could detect mercury in water in the range of 0.030–0.060 ppm, and ii) support onto which polyclonal antibodies were anchored and successfully bound to an osteosarcoma cell line expressing the target protein (TRIB2-GFP), as part of an immunoprecipitation assay.

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