- Publication . Article . 2021Open Access EnglishAuthors:Jordi Mayneris-Perxachs; José María Moreno-Navarrete; Marta Ballanti; Giovanni Monteleone; Omero Alessandro Paoluzi; Geltrude Mingrone; Philippe Lefebvre; Bart Staels; Massimo Federici; Josep Puig; +3 moreJordi Mayneris-Perxachs; José María Moreno-Navarrete; Marta Ballanti; Giovanni Monteleone; Omero Alessandro Paoluzi; Geltrude Mingrone; Philippe Lefebvre; Bart Staels; Massimo Federici; Josep Puig; Josep Garre; Rafael Ramos; José Manuel Fernández-Real;Publisher: Elsevier
Cell surface receptor-mediated viral entry plays a critical role in this infection. Well-established SARS-CoV-2 receptors such as ACE2 and TMPRSS2 are highly expressed in the gastrointestinal tract. In fact, there are evidences that SARS-CoV-2 infects epithelial cells from the digestive system. However, emerging research has identified novel mediators such as DPP9, TYK2, and CCR2, all playing a critical role in inflammation. We evaluated the expression of SARS-CoV-2 receptors in peripheral leukocytes (n=469), jejunum (n=30), and colon (n=37) of three independent cohorts by real-time PCR, RNA-sequencing, and microarray transcriptomics. We also performed HPCL-MS/MS lipidomics and metabolomics analyses to identify signatures linked to SARS-CoV-2 receptors. We found markedly higher peripheral leukocytes ACE2 expression levels in women compared to men, whereas the intestinal expression of TMPRSS2 was positively associated with BMI. Consistent lipidomics signatures associated with the expression of these mediators were found in both tissues and peripheral leukocytes involving n-3 long-chain PUFAs and arachidonic acid-derived eicosanoids, which play a key role in the regulation of inflammation and may interfere with viral entry and replication. Medium- and long-chain hydroxy acids, which have shown to interfere in viral replication, were also liked to SARS-CoV2 receptors. Gonadal steroids were also associated with the expression of some of these receptors, even after controlling for sex. The expression of SARS-CoV2 receptors was associated with several metabolic and nutritional traits in different cell types. This information may be useful in the design of potential therapies targeted at coronavirus entry. Graphical abstract
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- Publication . Article . 2021Open Access EnglishAuthors:Jordi Mayneris-Perxachs; José María Moreno-Navarrete; Marta Ballanti; Giovanni Monteleone; Omero Alessandro Paoluzi; Geltrude Mingrone; Philippe Lefebvre; Bart Staels; Massimo Federici; Josep Puig; +3 moreJordi Mayneris-Perxachs; José María Moreno-Navarrete; Marta Ballanti; Giovanni Monteleone; Omero Alessandro Paoluzi; Geltrude Mingrone; Philippe Lefebvre; Bart Staels; Massimo Federici; Josep Puig; Josep Garre; Rafael Ramos; José Manuel Fernández-Real;Publisher: Elsevier
Cell surface receptor-mediated viral entry plays a critical role in this infection. Well-established SARS-CoV-2 receptors such as ACE2 and TMPRSS2 are highly expressed in the gastrointestinal tract. In fact, there are evidences that SARS-CoV-2 infects epithelial cells from the digestive system. However, emerging research has identified novel mediators such as DPP9, TYK2, and CCR2, all playing a critical role in inflammation. We evaluated the expression of SARS-CoV-2 receptors in peripheral leukocytes (n=469), jejunum (n=30), and colon (n=37) of three independent cohorts by real-time PCR, RNA-sequencing, and microarray transcriptomics. We also performed HPCL-MS/MS lipidomics and metabolomics analyses to identify signatures linked to SARS-CoV-2 receptors. We found markedly higher peripheral leukocytes ACE2 expression levels in women compared to men, whereas the intestinal expression of TMPRSS2 was positively associated with BMI. Consistent lipidomics signatures associated with the expression of these mediators were found in both tissues and peripheral leukocytes involving n-3 long-chain PUFAs and arachidonic acid-derived eicosanoids, which play a key role in the regulation of inflammation and may interfere with viral entry and replication. Medium- and long-chain hydroxy acids, which have shown to interfere in viral replication, were also liked to SARS-CoV2 receptors. Gonadal steroids were also associated with the expression of some of these receptors, even after controlling for sex. The expression of SARS-CoV2 receptors was associated with several metabolic and nutritional traits in different cell types. This information may be useful in the design of potential therapies targeted at coronavirus entry. Graphical abstract
Average popularityAverage popularity In bottom 99%Average influencePopularity: Citation-based measure reflecting the current impact.Average influence In bottom 99%Influence: Citation-based measure reflecting the total impact.add Add to ORCIDPlease grant OpenAIRE to access and update your ORCID works.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.