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Genome-wide screens are a powerful technique to dissect the complex network of genes regulating diverse cellular phenotypes. The recent adaptation of the CRISPR-Cas9 system for genome engineering has revolutionized functional genomic screening. Here, we present protocols used to introduce Cas9 into human lymphoma cell lines, produce high-titer lentivirus of a genome-wide sgRNA library, transduce and culture cells during the screen, isolate genomic DNA, and prepare a custom library for next-generation sequencing. These protocols were tailored for loss-of-function CRISPR screens in human lymphoma cell lines but are highly amenable for other experimental purposes.

International audience; The corrosion process (oxidation and hydriding) of the zirconium alloy cladding is one of the limiting factors on the fuel rod lifetime, in particular for the Zircaloy-4 alloy. The corrosion rate of this alloy shows indeed a great acceleration at high burn-up in Light Water Reactors. Understanding the corrosion behavior under irradiation for this alloy is an important technological issue for the safety and efficiency of LWRs. In particular, understanding the effect of irradiation on the metal and the oxide layers is a key parameter in the study of corrosion behavior of zirconium alloys. Zircaloy-4 samples have undergone helium and proton ion-irradiation up to 0.3 dpa forming a uniform defect distribution up to 1 µm deep. Both as-received and pre-corroded samples have been irradiated in order to compare the effect of metal irradiation to that of oxide layer irradiation. After irradiation, samples have been corroded in order to study the impact of irradiation defects in the metal and in pre-existing oxide layers on the formation of new oxide layers. Synchrotron X-ray micro-diffraction and micro-fluorescence are used to follow the evolution of oxide crystallographic phases, texture and stoichiometry both in the metal and in the oxide in cross-section. In particular, the tetragonal oxide phase fraction, which has been known to have an important role on corrosion behavior, is mapped in both unirradiated and irradiated metal at the sub-micron scale and appears to be significantly affected by irradiation. These observations, complemented with electron microscopy analyses on samples in carefully chosen areas of interest are combined in order to fully characterize changes due to irradiation in metal and oxide phases of both alloys.

Part 2: Services and Interoperability; International audience; This paper aims to identify the need for Public Sector Information Systems (PSIS), a particular field of the IS discipline dealing with the design, redesign and evaluation of E-services in public sector organizations. A longitudinal and empirical study of an E-service project in a public sector organization in Bangladesh reveals that without addressing underlying organizational problems, taking account into organization contexts, statutes and practices and considering the organizational reality and users’ capability; it is difficult to implement E-services in public sector organizations. This paper argues that a particular information system (public sector information systems (PSIS)) has the potential to lead to better design and implementation of successful E-services in public sector organizations.

International audience; This chapter provides a critical analysis of the evidence for technical activity specialization in the European Upper Paleolithic by sex. It reviews the arguments based on the kind of evidence researchers are likely to collect (e.g., direct, indirect, and analogical). Some hypotheses are based on suppositions generated by ethnographic comparisons, while others rely on direct or indirect indices (task diversification, activity zone locations, skill level identification, diversity of grave goods, and body evidence like handprints and skeletons). The aim of this chapter is to show that there was a reasoned distribution of activities within groups, accompanied by an emerging social hierarchy, but that it is very difficult to account exactly for what women and men did. And even if we suspect that some tasks were respectively performed by males or females, it is possible that there was also a certain amount of technical specialization that was not related to gender.

International audience; This introductory chapter discusses the challenge of fostering sustainable development in Asia, where the economic, social and environmental contingencies are changing rapidly and concerns over, inter alia, climate change, environmental degradation, population growth and social deprivation provide for a complex terrain for decision-making. A number of perspectives that have been used in the extant literature to explain the specific societal role of business organisations in this region are critically reviewed and an emphasis is placed on their general lack of depth and contextualisation. This leads to the observation that this volume represents a timely contribution to our understanding of an understudied phenomenon: corporate sustainability-oriented practices in Asia. The book develops a collection of illustrative company cases that help us to clarify some conceptual and practical issues that surface the societal role of multinational companies in the Asian context. We hereby join a growing movement of research that seeks to unveil the conditions giving rise to a better and more context-sensitive way of integrating concerns for social and environmental impacts into corporate management decisions.

International audience; Orchestras of Digital Musical Instruments (DMIs) enable new musical collaboration possibilities, extending those of acoustic and electric orchestras. However the creation and development of these orchestras remain constrained. In fact, each new musical collaboration system or orchestra piece relies on a fixed number of musicians, a fixed set of instruments (often only one), and a fixed subset of possible modes of collaboration. In this paper, we describe a unified framework that enables the design of Digital Orchestras with potentially different DMIs and an expand-able set of collaboration modes. It relies on research done on analysis and classification of traditional and digital orchestras, on research in Collaborative Virtual Environments, and on interviews of musicians and composers. The BOEUF framework consists of a classification of modes of collaboration and a set of components for modelling digital orchestras. Integrating this framework into DMIs will enable advanced musical collaboration modes to be used in any digital orchestra, including spontaneous jam sessions.

International audience; This chapter introduces several state of the art techniques that could help to make deep underwater archaeological photogrammetric surveys easier, faster, more accurate, and to provide more visually appealing representations in 2D and 3D for both experts and public. We detail how the 3D captured data is analysed and then represented using ontologies, and how this facilitates interdisciplinary interpretation and cooperation. Towards more automation, we present a new method that adopts a deep learning approach for the detection and the recognition of objects of interest, amphorae for example. In order to provide more readable, direct and clearer illustrations, we describe several techniques that generate different styles of sketches out of orthophotos developed using neural networks. In the same direction, we present the Non-Photorealistic Rendering (NPR) technique, which converts a 3D model into a more readable 2D representation that is more useful to communicate and simplifies the identification of objects of interest. Regarding public dissemination, we demonstrate how recent advances in virtual reality to provide an accurate, high resolution, amusing and appropriate visualization tool that offers the public the possibility to ‘visit’ an unreachable archaeological site. Finally, we conclude by introducing the plenoptic approach, a new promising technology that can change the future of the photogrammetry by making it easier and less time consuming and that allows a user to create a 3D model using only one camera shot. Here, we introduce the concepts, the developing process, and some results, which we obtained with underwater imaging.

This chapter examines Leibniz’s complex relations to Descartes. These relations are deeply influenced by the evolution of the intellectual context from the beginning of the 1670s to the early eighteenth century. Beyond Leibniz’s overall appraisal of Descartes’s philosophy, there are three areas that stand out in which the discussion and criticism of Descartes’s ideas played a decisive role in the development of Leibniz’s thought: epistemology, natural philosophy, and philosophy of mind. There are three central issues at stake between the two philosophers: the nature and role of evidence, the use of final causes, and the Law of Continuity.