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  • image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
    Authors: Carrasco, Andres; Brown, Trecia A.; Lomber, Stephen G.;

    Assemblies of vertically connected neurons in the cerebral cortex form information processing units (columns) that participate in the distribution and segregation of sensory signals. Despite well-accepted models of columnar architecture, functional mechanisms of inter-laminar communication remain poorly understood. Hence, the purpose of the present investigation was to examine the effects of sensory information features on columnar response properties. Using acute recording techniques, extracellular response activity was collected from the right hemisphere of eight mature cats (felis catus). Recordings were conducted with multichannel electrodes that permitted the simultaneous acquisition of neuronal activity within primary auditory cortex columns. Neuronal responses to simple (pure tones), complex (noise burst and frequency modulated sweeps), and ecologically relevant (con-specific vocalizations) acoustic signals were measured. Collectively, the present investigation demonstrates that despite consistencies in neuronal tuning (characteristic frequency), irregularities in discharge activity between neurons of individual A1 columns increase as a function of spectral (signal complexity) and temporal (duration) acoustic variations. Multi-unit responses to acoustic signals within A1 columnsThe data set consists of eight multi-unit electrophysiology experiments located within a single .zip file. Acoustic feature (signal type and duration) are in subfolders where data rasters for each recording session conducted can be found. Columns represent time and rows trial number. Data is presented as Matlab files.DRYAD.zip

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    DANS-EASY
    Dataset . 2014
    Data sources: B2FIND
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      image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/ DANS-EASYarrow_drop_down
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      DANS-EASY
      Dataset . 2014
      Data sources: B2FIND
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  • image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
    Authors: Khan, Md. Nafiz Hasan; Neustaedter, Carman;

    In the near future, emergency services within Canada will be supporting new technologies for 9-1-1 call centres and firefighters to learn about an emergency situation. One such technology is drones. To understand the benefits and challenges of using drones within emergency response, we conducted a study with citizens who have called 9-1-1 and firefighters who respond to a range of everyday emergencies. Our results show that drones have numerous benefits to both firefighters and 9-1-1 callers which include context awareness and social support for callers who receive feelings of assurance that help is on the way. Privacy was largely not an issue, though safety issues arose especially for complex uses of drones such as indoor flying. Our results point to opportunities for designing drone systems that help people to develop a sense of trust with emergency response drones, and mitigate privacy and safety concerns with more complex drone systems. International audience

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    Other literature type . 2019
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    Hal-Diderot; Hal
    Conference object . Other literature type . 2019
    Data sources: Hal; Hal-Diderot
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      Hyper Article en Ligne
      Other literature type . 2019
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      Hal-Diderot; Hal
      Conference object . Other literature type . 2019
      Data sources: Hal; Hal-Diderot
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    Authors: Belle II Collaboration; Abudinén, F.; Adachi, I.; Adak, R.; +220 Authors

    We present the results of the re-discovery of the decay $B^0 \to \pi^- \ell^+ \nu_\ell$ in 34.6 fb$^{-1}$ of Belle II data using hadronic $B$-tagging via the Full Event Interpretation algorithm. We observe 21 signal events on a background of 155 in a fit to the distribution of the square of the missing mass, $M_{\mathrm{miss}}^2$, with a significance of 5.69$\sigma$, and determine a total branching fraction of (1.58 $\pm$ 0.43$_{\mathrm{stat}}$ $\pm$ 0.07$_{\mathrm{sys}}$) $\times 10^{-4}$.

    image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/ arXiv.org e-Print Ar...arrow_drop_down
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    arXiv.org e-Print Archive
    Other literature type . Preprint . 2020
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    Hal-Diderot
    Preprint . 2020
    Data sources: Hal-Diderot
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      image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/ arXiv.org e-Print Ar...arrow_drop_down
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      Other literature type . Preprint . 2020
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      Hal-Diderot
      Preprint . 2020
      Data sources: Hal-Diderot
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  • image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
    Authors: Peters, J, F; Dochviri, I, J;

    In the work using interval mathematics, we develop knowledge for cardinal numbers from the viewpoint of uncertainty analysis. In the finite non-T 1 topological spaces, the inclusion-exclusion formula provide interval estimations for the closure and interior of given sets. This paper introduces a novel approach that combines combinatorial and point-set topology, which leads to a number of results. Among these is the cardinality estimation for the intersection of two open sets that cover a hyperconnected topo-logical space.

    image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/ Halarrow_drop_down
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    Hal
    Preprint . 2020
    Data sources: Hal
    image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
    Hal-Diderot
    Other literature type . 2020
    Data sources: Hal-Diderot
    image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
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    Other literature type . 2020
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      Hal
      Preprint . 2020
      Data sources: Hal
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      Hal-Diderot
      Other literature type . 2020
      Data sources: Hal-Diderot
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      Hyper Article en Ligne
      Other literature type . 2020
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    Authors: Saravanan, Govindaraj; Krishnaswamy, N.;

    The present study on bibliometric analysis (cumulative) of seven ecology journals reveals that 21,118 authors have contributed 11394 papers during 2003 to 2012. The study examines the year-wise, institution-wise distribution of contributions, relative growth rate and doubling time, authorship patterns, prolific authors, Degree of collaboration, Collaborative co-efficient, etc. The study reveals that multiple authorship was dominant with 78.61% for the study period. The DC and CC were 0.78 and 0.54 respectively. The University Calif. Davis was found to be the top ranked institution contributing 370 papers. International audience

    image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/ Mémoires en Sciences...arrow_drop_down
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    Authors: David, Marion A.;

    Les lactones macrocycliques (LM) sont des anthelminthiques (AH) à effet paralysant très utilisés chez les animaux et les humains contre les nématodes parasites. Cependant, leur succès thérapeutique est compromis par la résistance croissante aux LM, qui pourrait être en partie dû aux ABC transporteurs P-glycoprotéines (Pgps) sélectionnés et surexprimés chez les nématodes résistants aux LM. Dans ce travail, nous avons étudié plus précisément la P-glycoprotéine 13 du parasite de petits ruminants, Haemonchus contortus. Son orthologue chez le modèle nématode C. elegans, Cel-Pgp-13, est exprimé dans les amphides, structures qui ont été associées à la sensibilité aux AH chez C. elegans et H. contortus. Pour prédire la capacité des Pgps de nematode à transporter des drogues, incluant des LM et autres AH, nous avons développé un modèle de docking in silico. Nous avons utilisé la structure cristallographique de C. elegans Pgp-1 (Cel-Pgp-1), et nous avons montré la liaison avec une forte affinité de plusieurs ligands décrits comme activateurs de sa fonction ATPasique. Nous avons aussi décrit une forte affinité des LM, et un site spécifique de liaison de ces composés à Cel-Pgp-1. Cette approche représente un outil important pour prédire les interactions entre AH, et pour concevoir rationnellement de nouveaux inhibiteurs compétitifs des Pgps de nématode, dans le but d'améliorer les stratégies thérapeutiques. Sur la base de cette approche, nous avons prédit la structure 3D de Hco-Pgp-13 à partir du cristal de Cel-Pgp-1 afin d'étudier son intéraction avec des substrats potentiels, en particulier les LM. Nous avons trouvé des affinités similaires pour différents composés précédemment testés sur Cel-Pgp-1. In vitro, la mesure de l'activité ATPasique montre que l'actinomycine D est un substrat de Hco-Pgp-13. Nos données démontrent la présence possible d'un domaine de reconnaissance multispécifique sur ce transporteur de parasite. La détermination par immunofluorescence de l'expression de Hco-Pgp-13 a montré une distribution tissulaire large indiquant que Hco-Pgp-13 pourrait jouer un role important dans le transport de substrats endogènes et/ou exogènes. En conclusion, ce travail permet de mieux comprendre le rôle des Pgps de nématodes dans le transport de médicaments AH, tant au niveau de l'organisme modèle C. elegans que du nématode parasite H. contortus. Cette étude suggère la conservation de la fonction de tranporteur ABC multidrogue dans ces espèces. La localisation de Hco-Pgp-13 sur les structures amphidiales, et son éventuelle implication dans la résistance aux médicaments et à la survie de H. contortus à l'exposition à des composés AH, restent à préciser. Macrocyclic lactones (ML) are paralyzing anthelmintics used in animals and humans against parasite nematodes. However, their therapeutic success is compromised by the spread of ML resistance. This might be at least partly due to P-glycoproteins (Pgps) ABC transporters that are selected and overexpressed in ML-resistant nematodes. Deciphering the role of the 10 Pgps expressed in the parasite of small ruminants Haemonchus contortus is thus of major importance to guaranty anthelmintic (AH) efficacy of various drugs. Here we focused on Hco-Pgp-13 due to the expression in the amphids of its closest ortholog in the model nematode C. elegans. Indeed, the amphids represent a putative entry route of drugs to reach AH targets in the nervous system and have been linked to AH susceptibility in C. elegans and H. contortus. In order to predict the capacity of nematode Pgps to transport drugs, including ML and otherAH, we have developed an in silico drug docking model. We have used C. elegans Pgp-1 (Cel-Pgp-1) crystal structure and have showed a high affinity binding of several ligands that have been shown to be activators of its ATPase function. ML were also found to bind with high affinity to Cel-Pgp-1, on a specific binding site. This approach provides a valuable tool to predict drug-drug interactions and to rationally design new competitive inhibitors of nematode Pgps, in order to improve anthelmintic therapeutics. We then predicted a putative 3D structure of Hco-Pgp-13 based on the recently released crystal of Cel-Pgp-1, with which it presented a high homology. This allowed the study of the interaction of Hco-Pgp-13 with potential substrates, in particular ML. We found similar affinities for various drugs previously tested on Cel-Pgp-1, supporting the good homology of these two proteins. Together with in vitro ATPase assay experiments that confirmed the substrate status of actinomycin D, this indicates a possible multispecifc recognition capacity of this parasitic transporter. The determination of Hco-Pgp-13 localization using immunohistochemistry showed a wide tissue expression consistent with a critical role for Hco-Pgp-13 in endogenous and/or exogenous substrate transport. In conclusion, this work provides insights into the role of nematode Pgps in transporting AH drugs, both at the level of the model organism C. elegans and of the parasitic nematode H. contortus. This suggests a high homology of function conserved between ABC tranporters in these species. The localization of such protein on amphidial structures and its possible involvement in drug resistance and survival of H. contortus to exposure to AH compounds remain to be precised.

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    Authors: Xu, Xingyuan; Moss, David, J;

    We investigate the application of integrated micro-combs in RF photonic systems and demonstrate a microwave photonic intensity differentiator based on a Kerr optical comb generated by a compact integrated micro-ring resonator. The on-chip Kerr optical comb is CMOS-compatible and contains a large number of comb lines, which can serve as a high-performance multi-wavelength source for the transversal filter, thus greatly reduce the cost, size, and complexity of the system. The operation principle is theoretically analyzed, and experimental demonstrations of fractional-, first-, second-, and thirdorder differentiation functions based on the principle are presented. International audience

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    Hal
    Preprint . 2021
    Data sources: Hal
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      Hal
      Preprint . 2021
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    Authors: Ben Ali, Maha; Gaudreault, Jonathan; D’amours, Sophie; Carle, Marc-André;

    "This paper proposes a demand fulfillment process for Make-To-Stock environments, integrating sales and operations planning (S&OP) and order promising, for a commodity market characterized by prices and demand seasonality. Considering differentiated customers, different products and multiple sourcing locations in a multi-period context, we define a multi-level decision framework in order to support short and medium term sales decisions in a way to maximize profits and to enhance the service level offered to high-priority customers. Our research exhibits three valuable elements: (1) we developed an order promising model based on nested booking limits and which allows order reassignment i.e. changing decisions of how firm orders have to be fulfilled (2) we used a rolling horizon simulation to evaluate performance of the demand fulfillment process proposed and (3) we compare it with common fulfillment processes such first-come first-served order processing. In order to evaluate the demand fulfillment process proposed, a numerical application based on softwood lumber manufacturers located in Eastern Canada is conducted and provides evidence that better performances (overall service level, high-priority service level and overall net profit) can be achieved by using nested booking limits and reviewing previous order promising decisions whilst respecting sales commitments." Colloque avec actes et comité de lecture. internationale. International audience

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    Other literature type . 2014
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    Hal-Diderot
    Other literature type . 2014
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      Other literature type . 2014
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      Other literature type . 2014
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    Authors: Sarkis, Carole;

    La croissance dendritique est calculée en utilisant un modèle champ de phase avec adaptation automatique anisotrope et non structurées d’un maillage éléments finis. Les inconnues sont la fonction champ de phase, une température adimensionnelle et une composition adimensionnelle, tel que proposé par [KAR1998] et [RAM2004]. Une interpolation linéaire d’éléments finis est utilisée pour les trois variables, après des techniques de stabilisation de discrétisation qui assurent la convergence vers une solution correcte non-oscillante. Afin d'effectuer des calculs quantitatifs de la croissance dendritique sur un grand domaine, deux ingrédients numériques supplémentaires sont nécessaires: un maillage adaptatif anisotrope et non structuré [COU2011], [COU2014] et un calcul parallèle [DIG2001], mis à disposition de la plateforme numérique utilisée (CimLib) basée sur des développements C++. L'adaptation du maillage se trouve à réduire considérablement le nombre de degrés de liberté. Les résultats des simulations en champ de phase pour les dendrites pour une solidification d'un matériau pur et d’un alliage binaire en deux et trois dimensions sont présentés et comparés à des travaux de référence. Une discussion sur les détails de l'algorithme et le temps CPU sont présentés et une comparaison avec un modèle macroscopique sont faite. Dendritic growth is computed using a phase-field model with automatic adaptation of an anisotropic and unstructured finite element mesh. Unknowns are the phase-field function, a dimensionless temperature and a dimensionless composition, as proposed by [KAR1998] and [RAM2004]. Linear finite element interpolation is used for all variables, after discretization stabilization techniques that ensure convergence towards a correct non-oscillating solution. In order to perform quantitative computations of dendritic growth on a large domain, two additional numerical ingredients are necessary: automatic anisotropic unstructured adaptive meshing [COU2011], [COU2014] and parallel implementations [DIG2001], both made available with the numerical platform used (CimLib) based on C++ developments. Mesh adaptation is found to greatly reduce the number of degrees of freedom. Results of phase-field simulations for dendritic solidification of a pure material and a binary alloy in two and three dimensions are shown and compared with reference work. Discussion on algorithm details and the CPU time are outlined and a comparison with a macroscopic model are made.

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    Hyper Article en Ligne
    Other literature type . 2016
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    Authors: Henry, Molly J.; Herrmann, Bjorn; Grahn, Jessica A.;

    Entrainment of neural oscillations on multiple time scales is important for the perception of speech. Musical rhythms, and in particular the perception of a regular beat in musical rhythms, is also likely to rely on entrainment of neural oscillations. One recently proposed approach to studying beat perception in the context of neural entrainment and resonance (the “frequency-tagging” approach) has received an enthusiastic response from the scientific community. A specific version of the approach involves comparing frequency-domain representations of acoustic rhythm stimuli to the frequency-domain representations of neural responses to those rhythms (measured by electroencephalography, EEG). The relative amplitudes at specific EEG frequencies are compared to the relative amplitudes at the same stimulus frequencies, and enhancements at beat-related frequencies in the EEG signal are interpreted as reflecting an internal representation of the beat. Here, we show that frequency-domain representations of rhythms are sensitive to the acoustic features of the tones making up the rhythms (tone duration, onset/offset ramp duration); in fact, relative amplitudes at beat-related frequencies can be completely reversed by manipulating tone acoustics. Crucially, we show that changes to these acoustic tone features, and in turn changes to the frequency-domain representations of rhythms, do not affect beat perception. Instead, beat perception depends on the pattern of onsets (i.e., whether a rhythm has a simple or complex metrical structure). Moreover, we show that beat perception can differ for rhythms that have numerically identical frequency-domain representations. Thus, frequency-domain representations of rhythms are dissociable from beat perception. For this reason, we suggest caution in interpreting direct comparisons of rhythms and brain signals in the frequency domain. Instead, we suggest that combining EEG measurements of neural signals with creative behavioral paradigms is of more benefit to our understanding of beat perception. single participant behavioral data files.mat files for single participants. README.txt file in each zipped folder describes columnsdryad_data.zip

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    DANS-EASY
    Dataset . 2017
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      DANS-EASY
      Dataset . 2017
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174 Research products
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    Authors: Carrasco, Andres; Brown, Trecia A.; Lomber, Stephen G.;

    Assemblies of vertically connected neurons in the cerebral cortex form information processing units (columns) that participate in the distribution and segregation of sensory signals. Despite well-accepted models of columnar architecture, functional mechanisms of inter-laminar communication remain poorly understood. Hence, the purpose of the present investigation was to examine the effects of sensory information features on columnar response properties. Using acute recording techniques, extracellular response activity was collected from the right hemisphere of eight mature cats (felis catus). Recordings were conducted with multichannel electrodes that permitted the simultaneous acquisition of neuronal activity within primary auditory cortex columns. Neuronal responses to simple (pure tones), complex (noise burst and frequency modulated sweeps), and ecologically relevant (con-specific vocalizations) acoustic signals were measured. Collectively, the present investigation demonstrates that despite consistencies in neuronal tuning (characteristic frequency), irregularities in discharge activity between neurons of individual A1 columns increase as a function of spectral (signal complexity) and temporal (duration) acoustic variations. Multi-unit responses to acoustic signals within A1 columnsThe data set consists of eight multi-unit electrophysiology experiments located within a single .zip file. Acoustic feature (signal type and duration) are in subfolders where data rasters for each recording session conducted can be found. Columns represent time and rows trial number. Data is presented as Matlab files.DRYAD.zip

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    DANS-EASY
    Dataset . 2014
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      DANS-EASY
      Dataset . 2014
      Data sources: B2FIND
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    Authors: Khan, Md. Nafiz Hasan; Neustaedter, Carman;

    In the near future, emergency services within Canada will be supporting new technologies for 9-1-1 call centres and firefighters to learn about an emergency situation. One such technology is drones. To understand the benefits and challenges of using drones within emergency response, we conducted a study with citizens who have called 9-1-1 and firefighters who respond to a range of everyday emergencies. Our results show that drones have numerous benefits to both firefighters and 9-1-1 callers which include context awareness and social support for callers who receive feelings of assurance that help is on the way. Privacy was largely not an issue, though safety issues arose especially for complex uses of drones such as indoor flying. Our results point to opportunities for designing drone systems that help people to develop a sense of trust with emergency response drones, and mitigate privacy and safety concerns with more complex drone systems. International audience

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    Other literature type . 2019
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    Hal-Diderot; Hal
    Conference object . Other literature type . 2019
    Data sources: Hal; Hal-Diderot
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      Other literature type . 2019
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      Conference object . Other literature type . 2019
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    Authors: Belle II Collaboration; Abudinén, F.; Adachi, I.; Adak, R.; +220 Authors

    We present the results of the re-discovery of the decay $B^0 \to \pi^- \ell^+ \nu_\ell$ in 34.6 fb$^{-1}$ of Belle II data using hadronic $B$-tagging via the Full Event Interpretation algorithm. We observe 21 signal events on a background of 155 in a fit to the distribution of the square of the missing mass, $M_{\mathrm{miss}}^2$, with a significance of 5.69$\sigma$, and determine a total branching fraction of (1.58 $\pm$ 0.43$_{\mathrm{stat}}$ $\pm$ 0.07$_{\mathrm{sys}}$) $\times 10^{-4}$.

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    arXiv.org e-Print Archive
    Other literature type . Preprint . 2020
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    Preprint . 2020
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      Other literature type . Preprint . 2020
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    Authors: Peters, J, F; Dochviri, I, J;

    In the work using interval mathematics, we develop knowledge for cardinal numbers from the viewpoint of uncertainty analysis. In the finite non-T 1 topological spaces, the inclusion-exclusion formula provide interval estimations for the closure and interior of given sets. This paper introduces a novel approach that combines combinatorial and point-set topology, which leads to a number of results. Among these is the cardinality estimation for the intersection of two open sets that cover a hyperconnected topo-logical space.

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      Hal
      Preprint . 2020
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      image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
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      Other literature type . 2020
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    Authors: Saravanan, Govindaraj; Krishnaswamy, N.;

    The present study on bibliometric analysis (cumulative) of seven ecology journals reveals that 21,118 authors have contributed 11394 papers during 2003 to 2012. The study examines the year-wise, institution-wise distribution of contributions, relative growth rate and doubling time, authorship patterns, prolific authors, Degree of collaboration, Collaborative co-efficient, etc. The study reveals that multiple authorship was dominant with 78.61% for the study period. The DC and CC were 0.78 and 0.54 respectively. The University Calif. Davis was found to be the top ranked institution contributing 370 papers. International audience

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