Supplemental material, Supplementary_figure_2 for Bicyclic eremophilane-type petasite sesquiterpenes potentiate peroxisome proliferator–activated receptor γ activator–mediated inhibition of dendritic cells by Narcy Arizmendi, Chenjie Hou, Fujiang Guo, Yiming Li and Marianna Kulka in International Journal of Immunopathology and Pharmacology
During EAE, surfen increases lymph node cell count, but reduces proliferation in extracted CD4 positive T cells. Superficial cervical, axillary, brachial and inguinal lymph nodes were pooled from individual mice (n shown). Total cells were counted (a) and then isolated T cells were stimulated in vivo with anti-CD3, anti CD-28 T cell expander beads for 24 h, and proliferation assessed by Oregon Green staining (b). Spleens were also homogenized (n indicates number of mice, with one spleen per mouse) and cells stained with antibodies directed against surface markers and then analyzed by flow cytometry. The percentage of CD4 positive T cells among the extracts is shown (c) along with the gating strategy (d). Data compare EAE treated with vehicle (EAE-V) or surfen (EAE-S), and is shown as mean ± SEM. Significance compares surfen with vehicle (* = P
Project: CIHR , NWO | XCiDE: Crossing the Combu... (2300153186)
Figure S1. The genotype of NOD1/2 DKO mice. Genomic DNA from mice was amplified by PCR with specific primers to detect the disrupted sequences on a 1% agarose gel with a 100Â bp marker. First 5 bands are the KO mice and the last bands are the WT mice in A and B. NÂ =â 5/3 per group. (TIFF 12868 kb)
Role of pslB gene in PAPI-1 transfer. Results were shown as mean ± SD for three independent replicates. Statistical significance was analyzed by One-way ANOVA compared to the positive control (ns: no significant difference; and *** p
Differentially methylated sites unique to ME/CFS GC-Typical. Epigenetic loci that are specifically differentially methylated in the ME/CFS GC-Typical subgroup (p ≤ 0.05, Beta-difference ≥ 5%, after 10 000 permutations). (XLSX 24 kb)
Additional file 2: Figure S2. Biophysical response of uninfected red blood cells to anti-malarial compounds. The anti-malarial compounds tested in this study did not significantly affect the deformability of uninfected RBCs.