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At the Faculty of Agriculture and Life Sciences of Maribor we made an experiment in which the possibilities of multiplying potted and garden hydrangeas in tissue culture were studied. The shoots were sterilized according to four different methods: with sodium hypochlorite 2 min (sterilization S1), with sodium hypochlorite 10 min (sterilization S2), with dichloroisocyanuric acid in combination with silver nitrate 2 min (sterilization S3) and with dichloroisocyanuric acid in combination with silver nitrate 10 min (sterilization S4). The sterilization S3 with which we managed to successfully sterilize 81,82 % of the shoots proved the best. The successfully sterilized shoots were then transferred to five different nurseries for their regeneration (G1, G2, G3, G4 and G5), which differed according to the basic nursery (MS resp. McCown) as well as according to the growth regulators. Among the five nurseries used, it was the nursery G5 (based on McCown) that showed the best results with a multiplication factor of 1,33 after five weeks, while in the other nurseries (G1, G2, G3 and G4) we did not succeed in multiplying any of the shoots. The sufficiently developed shoots were then transferred to two rooting nurseries (K1 and K2), which differed only in their auxin contents (IBA and NAA). The shoots of garden hydrangeas did better in the nursery K2, which contained 0,5 mg/l NAA and where the rooting capacity reached from 50 to 62,50 %. The shoots of potted hydrangeas succeeded better in the nursery K1, containing 0,5 mg/l IBA and where a rooting capacity from 53,33 to 100 % was reached. Na Fakulteti za kmetijstvo in biosistemske vede v Mariboru smo opravili poskus, v katerem smo proučevali možnosti razmnoževanja lončnih in vrtnih hortenzij v tkivni kulturi. Poganjke smo sterilizirali po štirih metodah: z natrijevim hipokloritom 2 min (sterilizacija S1), natrijevim hipokloritom 10 min (sterilizacija S2), z dikloroizocianurno kislino v kombinaciji s srebrovim nitratom 2 min (sterilizacija S3) in z dikloroizocianurno kislino v kombinaciji s srebrovim nitratom 10 min (sterilizacija S4). Kot najboljša se je izkazala sterilizacija S3, s katero smo uspešno sterilizirali 81,82 % poganjkov. Uspešno sterilizirane poganjke smo prenesli na pet različnih gojišč za regeneracijo (G1, G2, G3, G4 in G5), ki so se razlikovala po osnovnem gojišču (MS oz. McCown) in po vsebnosti rastnih regulatorjev. Med petimi uporabljenimi gojišči, se je gojišče G5 (ki je baziralo na McCown) izkazalo kot najboljše in sicer je namnožitveni faktor po petih tednih znašal 1,33, pri ostalih (G1, G2, G3 in G4) pa nismo uspeli namnožiti nobenega poganjka. Dovolj razvite poganjke smo prestavili na dve gojišči za koreninjenje (K1 in K2), ki sta se razlikovali po vsebnosti avksina (IBA ali NAA). Poganjki vrtne hortenzije so bolje koreninili na gojišču K2, ki je vsebovalo 0,5 mg/l NAA, kjer smo dosegli med 50 in 62,50 % ukoreninjenost. Poganjki lončne hortenzije so bolje koreninili na gojišču K1, ki je vsebovalo 0,5 mg/l IBA in smo dosegli med 53,33 in 100 % ukoreninjenost.

Background and aims We have previously found increased levels of the cysteine protease legumain in plasma and plaques from patients with carotid atherosclerosis. This study further investigated legumain during acute cardiovascular events. Methods Circulating levels of legumain from patients and legumain released from platelets were assessed by enzyme-linked-immunosorbent assay. Quantitative PCR and immunoblotting were used to study expression, while localization was visualized by immunohistochemistry. Results In the SUMMIT Malmö cohort (n = 339 with or without type 2 diabetes and/or cardiovascular disease [CVD], and 64 healthy controls), the levels of circulating legumain were associated with the presence of CVD in non-diabetics, with no relation to outcome. In symptomatic carotid plaques and in samples from both coronary and intracerebral thrombi obtained during acute cardiovascular events, legumain was co-localized with macrophages in the same regions as platelets. In vitro, legumain was shown to be present in and released from platelets upon activation. In addition, THP-1 macrophages exposed to releasate from activated platelets showed increased legumain expression. Interestingly, primary peripheral blood mononuclear cells stimulated with recombinant legumain promoted anti-inflammatory responses. Finally, in a STEMI population (POSTEMI; n = 272), patients had significantly higher circulating legumain before and immediately after percutaneous coronary intervention compared with healthy controls (n = 67), and high levels were associated with improved outcome. Conclusions Our data demonstrate for the first time that legumain is upregulated during acute cardiovascular events and is associated with improved outcome.

Publisher Copyright: © 2015, Versalius University Medical Publisher. All rights reserved. The study represents a comprehensive retrospective morphological and immunohistochemical profiling of pancreatic neuroendocrine neoplasms (PNENs) in order to reveal the associations between morphological and molecular parameters. The local tumour spread (T), presence of metastases in regional lymph nodes (N) and distant organs (M), tumour grade (G) and resection line status (R) by pathology findings (pTNMGR), mitotic activity, perineural, vascular and lymphatic invasion were assessed in 16 surgically resected PNENs. By immunohistochemistry, expression of Ki-67, p53, p27, p21, cyclin D1, Bcl-2, E-cadherin, CD44, vimentin, cyclooxygenase 2 (COX-2), microvascular density, and cytokeratin (CK) spectrum, along with neuroendocrine, intestinal and squamous markers were detected. Descriptive statistics, Chi-square test, Spearman’s rank correlation, Mann–Whitney and Kruskal–Wallis methods were applied; p < 0.05 was considered significant. Ki-67, CK19, p63, vimentin and COX-2 were significantly up-regulated in PNENs in comparison to benign pancreatic islets. A complex network of morphological and molecular associations was identified. Ki-67 correlated with PNEN size (p = 0.022), the World Health Organization 2004 and 2010 classification grades (p = 0.021 and p = 0.002), stage (p = 0.028) and mitotic count (p = 0.007) but among molecular markers – with CK19 (p = 0.033) and vimentin (p = 0.045). CK19 was significantly up-regulated in PNENs, having higher pT (p = 0.018), pR (p = 0.025), vascular (p = 0.020), perineural (p = 0.026) and lymphatic invasion (p = 0.043). In conclusion, proliferation activity (by Ki-67), E-cadherin, vimentin and CK19 are important molecular characteristics of PNENs due to significant associations with morphological tumour characteristics, pTNMGR and invasive growth. Peer reviewed

In this context, the overall purpose of this review is to bring attention to the opportunities that five countries in the region - Bangladesh, India, Nepal, Pakistan and Sri Lanka - have to strengthen and expand interventions to improve the reproductive health of poor women. The specific objectives are: i) to provide an accurate picture of the current status of women's reproductive health, describe the use of reproductive health services and barriers to use, and identify the improvements required to increase their effectiveness and improve health outcomes; ii) to elucidate individual and household characteristics that affect reproductive health status and use of services so that the most important of these can be used to identify women and households with the greatest need for care to achieve better health; iii) to describe a simple and effective approach - decentralized action planning - that can be used widely in all five countries to improve reproductive health service delivery and outcomes, and point to a body of best practices in reproductive health that provides models and lessons for improvements in South Asia; and iv) to strengthen the case for investing in poor women's reproductive health by demonstrating the links between poverty, inequality, reproductive health care and expenditure.

The indicators, which were piloted in Senegal, provide a set of metrics to benchmark the performance of schools and health clinics in Africa. The indicators can be used to track progress within and across countries over time, and aim to enhance active monitoring of service delivery to increase public accountability and good governance. The service delivery indicators project takes as its starting point the literature on how to boost education and health outcomes in developing countries. This literature shows robust evidence that the type of individuals attracted to specific tasks at different levels of the service delivery hierarchy, as well as the set of incentives they face to actually exert effort, are positively and significantly related to education and health outcomes. To evaluate the feasibility of the indicators, pilot surveys in primary education and health care were implemented in Senegal in 2010. The results from the pilot studies demonstrate that the indicators methodology is capable of providing the necessary information to construct harmonized indicators on the quality of service delivery, as experienced by the citizen, using a single set of instruments at a single point of collection. This paper is structured as follows: section one gives introduction. Section two outlines the analytical underpinnings of the indicators and how they are categorized. Section three presents the methodology of the pilot surveys in Senegal. The results from the pilot are presented and analyzed in section four. Section five presents results on education outcomes, as evidenced by student test scores. Section six discusses the advantages and disadvantages of collapsing the indicators into one score or index, and provides a method for doing so in case such an index is deemed appropriate. Section seven discusses lessons learned, trade-offs, and options for scaling up the project.

The essential oil obtained by hydrodistillation from fresh leaves of Casearia lasiophylla Eichler, Salicaceae, was analyzed by gas capillary (GC/FID and GC/MS). The cytotoxicity of the leaves essential oil was tested in vitro againstU251 (glioma), UACC-62 (melanoma), MCF-7 (breast), NC1-ADR/RES (ovarian-resistant), NCI-H460 (lung), PC03 (prostate), OVCAR-3 (ovarian), HT-29 (colon) and K562 (leukemia) human cancer cells and against VERO (no cancer cell). The yield of oil was 0.02%. Fifty two compounds were identified, representing 87.1% of the total of the oil. The main components were identified as germacrene D (18.6%), β-caryophyllene (14.7%), δ-cadinene (6.2%), and α-cadinol (5.4%). The oil exhibited antiproliferative activity against all cell lines (TGI<100 µg/mL), with exception of NCI-H460 cell line (TGI 191.31 µg/mL). The highest activity was observed against UACC-62 (TGI 7.30 µg/mL), and K562 (TGI 7.56 µg/mL) cell lines. The observed activity could be related to high content of germacrene D and β-caryophyllene, compounds known as cytotoxic.

TGF-beta1 is a pluripotent cytokine with diverse effects in the normal development of mammary glands, and in the development of malignant tumors of the breast. The aim of the study was to determine the levels of TGF-beta1 in the group of advanced breast cancer, in which increased TGF-beta1 levels were most likely to be expected. TGF-beta1 levels were also compared with estradiol levels. Our results suggested that TGF-beta1 synthesis may be regulated by estrogen or anti-estrogen through ER. Finding of increased TGF-beta1 levels, due to its possible role in predicting invasive phenotype in later phases of tumor progression, may indicate the tendency of tumor tissue towards autonomy.

The HELLP (hemolysis, elevated liver enzymes, and low platelets) syndrome is a severe and life-threatening complication of preeclampsia with typical laboratory findings. The frequency of the disease is 1 to 150-300 live births in perinatal centers. The course of the HELLP syndrome is unpredictable. On the one hand, complete reversal of symptoms under conservative treatment have been reported in individual cases, on the other hand, rapid, therapy-resistant deterioration of the disease had been observed in the majority of patients accompanied by severe complications as liver rupture. As a consequence; the mother and the newborn need intensive care and these women should be delivered in an obstetric intensive care unit. The maternal mortality reported from the international literature is 3.3% and the perinatal mortality 22.6%. This review will emphasize the controversies surrounding the diagnosis and management of HELLP syndrome. HELLP (hemoliz, yükselmiş karaciğer enzimleri ve düşük trombosit) sendromu tipik laboratuar bulguları olan, şiddetli ve hayatı tehdit eden bir preeklampsi komplikasyonudur. Perinatal merkezlerde hastalığın sıklığı 150-300 canlı doğumda 1'dir. HELLP sendromunun seyri tahmin edilemez. Bir tarafta tedavi ile semptomların tam olarak geri döndüğü az sayıdaki vakalar bildirilirken, diğer tarafta hastaların çoğunda karaciğer rüptürü gibi şiddetli komplikasyonların eşlik ettiği, hızlı ve tedaviye dirençli bir kötüye gidiş gözlenmiştir. Sonuç olarak anne ve yenidoğanın yoğun bakıma ihtiyacı vardır ve bu kadınlar bir obstetrik yoğun bakım ünitesinde doğum yapmalıdır. Uluslararası literatürde maternal mortalite %3,3 ve perinatal mortalite %22,6 olarak bildirilmiştir. Bu derleme HELLP sendromunun tanı ve tedavisi ile ilgili tarışmaların üzerinde duracaktır.

O sistema de seguro de saúde atual é um modelo baseado na probabilidade de ocorrência de uma doença. Toda a análise financeira é realizada para calcular o quanto o beneficiário do plano de saúde custará à empresa em caso de doença grave. Nesse cenário, grandes grupos podem oferecer conjuntos de risco maiores e mais estáveis, permitindo às seguradoras cobrar menos. Mas o seguro de saúde não deve "assegurar" a saúde? Não deveria promover a saúde em vez de calcular o preço da doença? Promoção da saúde é um processo que engloba o bem-estar físico, mental e social. Além disso, exige uma abordagem multidisciplinar para o seu desenvolvimento. As grandes empresas entendem que promover a saúde dos trabalhadores, através de programas de bem-estar centrados no ambiente de trabalho, é o caminho correto para ajudar as pessoas as serem mais saudáveis, mais produtivas e, além disso, há um retorno positivo do investimento. No entanto, as pequenas empresas, que empregam mais de metade da força de trabalho, não possuem programas de promoção da saúde estabelecidos para seus colaboradores. A questão que impulsiona este estudo é: qual deve ser o design de um novo modelo de negócio de seguros de saúde, centrado em empregados de pequenas empresas, que promova simultaneamente a saúde e seja economicamente viável? O presente trabalho responde esta proposição com a criação de um novo conceito de modelo de negócios de assistência de saúde, através do Cyber-Physical System (CPS) e design de serviços. Today's health insurance systems is a model based on the risk of becoming sick. All the financial analysis is done to calculate how much the beneficiary of the health plan will cost to the company in case of a severe illness. In this scenario, large groups can offer more extensive and more stable risk pools, permitting insurers to charge less. However, should not health insurance "ensure" health? Should not it promote health rather than calculate the price of the disease? Health Promotion is a process and encompasses the physical, mental and social well-being. Besides, demands a multidisciplinary approach to its development. Big companies have understood that promoting workers health, through workplace wellness programs, is the right path to have healthier people been more productive and there is a positive return on investment instead. However, small business, that employs more than a half of the work-force do not have established health promotion programs for their members. The question that drives this study is: what should be the design of a new health insurance business model, centered in small businesses employees, that simultaneously promotes health and be economically viable? The present work will respond this proposition with a creation of a new concept of a health assistance business model, through cyber-physical systems (CPS) and service design. Dissertação (mestrado) - Universidade Federal do ABC, Programa de Pós-Graduação em Engenharia e Gestão da Inovação, Santo André, 2018. Coorientador: Prof. Dr. Anderson Orzari Ribeiro Orientadora: Profa. Dra. Luciana Pereira

Lung transplantation in patient with pulmonary alveolar microlithiasis - a case report.