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Belgium Herbarium image of Meise Botanic Garden.

Effects of prolonged in vitro culture expansion on DPPC ageing characteristics. (A) Digital images representing the extent of senescence-associated β-galactosidase staining by DPPCs at early PDs (7-8 PDs) and towards the end of their respective proliferative lifespans (A1 at 23 PDs, A3 at 83 PDs and B1 at 34 PDs). At 7-8 PDs, all DPPCs were morphologically similar, with the characteristic MSC morphology and were negative for β-galactosidase staining. At PDs towards the later stages of their respective proliferative lifespans, higher percentages of cells stained positive for β-galactosidase, which were larger, more stellate-like and with prominent stress fibres. Scale bars = 100 µm. (B) Analysis of cellular surface area throughout the proliferative lifespan of A1, A3 and B1, using ImageJ® Software (average ± SE, **p<0.001). Significant increases in each DPPC population’s surface area were identified with proliferative lifespan. Only when high proliferative capacity, A3, reached senescence (85 PDs) were surface areas non-significantly different to senescent A1 populations at 22 PDs (p>0.05). Senescent B1 surface areas at 35 PDs did not reach the cell area sizes of senescent A1 or A3 at 22 PDs and 85 PDs, respectively. (C) Gene expression of the MSC markers, CD73, CD90 and CD105, was gradually lost during in vitro expansion. CD105 was lost initially, based on expression levels in A1 (11 PDs) and A3 (24 PDs), whilst CD90 and CD73 expression was subsequently lost towards the end of each population’s respective proliferative lifespans. Whilst the premature loss of CD105 was not as apparent with B1, all three markers were lost towards the end of its proliferative lifespan (24 PDs). NA indicates that no cells were available for analysis at these PDs. β-actin served as the reference housekeeping gene.

The presentation corresponds to a IUPAC WorldFair Chemistry Webinar. The presentation aims at presenting in 5mn the type of chemistry data that are used in astrophysics, how we annotate those data, how we manipulate those data, what are the challenges and the issues linked to FAIR implementation, and what could be the collaboration with IUPAC. The PDF are uploaded at DOI 10.5281/zenodo.7101252 IUPAC : https://iupac.org/worldfair-global-cooperation-on-fair-data-policy-and-practice/ and https://iupac.org/project/2022-012-1-024/ VAMDC: https://vamdc.org

The dataset originates from an analysis of the long-term records of failures of a water distribution network (WDN) which provides the water supply in a town of about 30 000 inhabitants. The town is situated in Central Europe and covers approximately 24 km2. The failure records available covered the period from 2000 to 2016. The information available are not directly about WDN failures, but only about the repairs performed, accessible in the form of an aggregate record which included the number of repairs per calendar month. The dataset is based on the simplified assumption that the number of recorded repairs equals the number of failures which occurred during a given month. Information on the specific times at which individual failures occurred are not available. The numbers of failures recorded in this dataset are derived from the numbers of repairs performed. These repairs involved not only pipe breaks, but also all cases when the relevant WDN lost its ability to perform as required, e.g. pump failures and failures occurring when operating stop valves, water flow meters and other WDN parts.

Additional file 4:Figure S4. Correlations between ZMAT1 with key nodes of cell cycle and apoptosis. (A) Correlations between ZMAT1 with Cyclin Dependent Kinases (CDKs) and cyclins in TCGA data. (B) Correlations between ZMAT1 with apoptosis modulators in TCGA data.

Apodemus flavicollis (Yellow-necked mouse) from Zoological collection of the Mammal Research Institute, PAS. Collected 1967-11-16, in: Białowieża National Park, Poland. Material type:

Belgium Herbarium image of Meise Botanic Garden.

Belgium Herbarium image of Meise Botanic Garden.

This find is registered at Portable Antiquities of the Netherlands with number PAN-00055241