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  • image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
    Authors: De Méritens, Patrice; Fitoussi, Jean-Paul;
    image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/ SPIRE - Sciences Po ...arrow_drop_down
    image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
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      image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/ SPIRE - Sciences Po ...arrow_drop_down
      image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
  • image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
    Authors: Miras, Claude de;
    image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/ Horizon / Pleins tex...arrow_drop_down
    image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
    Horizon / Pleins textes
    Other literature type . 1978
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      image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/ Horizon / Pleins tex...arrow_drop_down
      image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
      Horizon / Pleins textes
      Other literature type . 1978
  • image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
    Authors: van Chuc Nguyen, ?.; Dandach, A.; Thi Thu Ha Vu, ?.; Fongarland, P.; +1 Authors

    Abstract Tungstated zirconia, ZrW, is reported as an efficient solid acid catalyst in the conversion of cellulose into lactic acid in hydrothermal conditions. The active phase of ZrW is generally ascribed to more or less polymerized WOx domains dispersed on zirconia. In this study, commercial uncalcined and calcined tungstated zirconia were used as catalysts for the conversion of cellulose under hydrothermal conditions at 190 °C. Their activities were also compared with uncalcined zirconium oxyhydroxide, ZrO2-x(OH)2x and calcined zirconia, ZrO2. Contrary to the expected results, it is disclosed that uncalcined ZrW exhibits higher catalytic activity and stability than calcined ZrW. Modifications in the products distribution were observed as glycolic acid formation at the expense of lactic acid formation upon ZrW calcination. Even more surprising, the catalytic activity of W free amorphous zirconium oxyhydroxide ZrO2-x(OH)2x is similar to uncalcined ZrW, with lactic acid as the main product while ZrO2 does not have any catalytic activity. These results suggest that the active phase of these catalysts might combine the couple Zr4+ and OH− species while the role of W species would be of secondary importance.

    image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/ Molecular Catalysisarrow_drop_down
    image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
    image/svg+xml Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao Closed Access logo, derived from PLoS Open Access logo. This version with transparent background. http://commons.wikimedia.org/wiki/File:Closed_Access_logo_transparent.svg Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao
    Molecular Catalysis
    Article . 2019
    License: Elsevier TDM
    Data sources: Crossref
    Hal-Diderot
    Article . 2019
    License: CC BY NC
    Data sources: Hal-Diderot
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      image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/ Molecular Catalysisarrow_drop_down
      image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
      image/svg+xml Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao Closed Access logo, derived from PLoS Open Access logo. This version with transparent background. http://commons.wikimedia.org/wiki/File:Closed_Access_logo_transparent.svg Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao
      Molecular Catalysis
      Article . 2019
      License: Elsevier TDM
      Data sources: Crossref
      Hal-Diderot
      Article . 2019
      License: CC BY NC
      Data sources: Hal-Diderot
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  • image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
    Authors: Cécile Mons; Thomas Botzanowski; Anton Nikolaev; Petra Hellwig; +4 Authors

    International audience; Human mitoNEET (mNT) is the first identified Fe−S protein of the mammalian outer mitochondrial membrane. Recently, we demonstrated the involvement of mNT in a specific cytosolic pathway dedicated to the reactivation of oxidatively damaged cytosolic aconitase by cluster transfer. In vitro studies using apo-ferredoxin (FDX) reveal that mNT uses an Fe-based redox switch mechanism to regulate the transfer of its cluster. Using the "gold standard" cluster recipient protein, FDX, we show that this transfer is direct and that only one of the two mNT clusters is transferred when the second one is decomposed. Combining complementary biophysical and biochemical approaches, we show that pH affects both the sensitivity of the cluster to O 2 and dimer stability. Around physiological cytosolic pH, the ability of mNT to transfer its cluster is tightly regulated by the pH. Finally, mNT is extremely resistant to H 2 O 2 compared to ISCU and SufB, two other Fe−S cluster transfer proteins, which is consistent with its involvement in a repair pathway of stress-damaged Fe−S proteins. Taken together, our results suggest that the ability of mNT to transfer its cluster to recipient proteins is not only controlled by the redox state of its cluster but also tightly modulated by the pH of the cytosol. We propose that when pathophysiological conditions such as cancer and neurodegenerative diseases dysregulate cellular pH homeostasis, this pH-dependent regulation of mNT is lost, as is the regulation of cellular pathways under the control of mNT. I ron−sulfur (Fe−S) clusters are evolutionarily ancient and highly conserved prosthetic cofactors. Composed of only iron and sulfur, they are involved in many essential biological processes. 1,2 MitoNEET (mNT), also known as CISD1, is the first identified Fe−S protein of the mammalian outer mitochondrial membrane (OMM). 3,4 This is a small homodimeric protein (13 kDa for each monomer) anchored to the OMM by its 32-amino acid N-terminus with the major part of the protein, including the C-terminal Fe−S binding domain, located in the cytosol. 4 Each monomer accommodates one [2Fe-2S] cluster coordinated by three cysteines (C72, C74, and C83) and one histidine (H87) in a CDGSH domain 5−8 as other members of the NEET protein family, 9 which also includes Miner1 (or CISD2) and Miner2 (or CISD3) in mammals. 10 Although the biological activity of mNT is still debated, 11 studies have shown that it is involved in the regulation of iron/reactive oxygen species homeo-stasis, 12−14 in the regulation of lipid and glucose metabolism , 13,15 and in cell proliferation in breast cancer. 16 In vitro studies revealed that holo-mNT (the form of the protein with the cluster) is able to transfer its Fe−S cluster to very diverse apoprotein (an Fe−S protein, which has lost its cluster) recipients assembling either a [2Fe-2S] cluster as ferredoxin from various organisms, 17,18 human anamorsin 19 and CISD2, 20 or a [4Fe-4S] cluster as mammalian iron regulatory protein-1 (IRP-1)/cytosolic aconitase (c-aconi-tase). 14 On the basis of in cellulo experiments, we showed that mNT is able to repair the oxidatively damaged Fe−S cluster of human IRP-1/c-aconitase by transferring its cluster to the damaged protein. 14 Recently, we started to investigate in depth the in vitro cluster transfer reaction, focusing on the transfer from holo-mNT to [2Fe-2S] recipient protein. We unambiguously demonstrated that oxidized mNT ([2Fe-2S] 2+) triggers cluster transfer, whereas reduction of its cluster abrogates this transfer. Moreover, while O 2 significantly affects the lability of the oxidized mNT cluster, it does not interfere with the cluster

    image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/ Biochemistry; Hal-Di...arrow_drop_down
    image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
    Biochemistry
    Article . 2018
    Data sources: Crossref
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  • image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
    Authors: Ferreira Torres, Luana; Dechamp, Eveline; Costa Alves, Gabriel Sergio; Marraccini, Pierre; +2 Authors

    O objetivo deste estudo foi obter uma visão geral dos genes ativos em folhas de café arábica variedade Caturra quando submetido a vários estresses abióticos e analisar estes genes identificados in silico por meio da técnica de RT-qPCR. O material vegetal consistiu de plantas in vitro de café arábica expostos por 3 horas sob os diferentes estresses: seca - baixa umidade relativa 9%, frio 5°C, calor 40°C, alta intensidade luminosa 200 μM m-2 s-1 e aplicação exógena de ácido abscísico 10 μM. Após o período de estresse, as folhas de café foram coletadas para a extração de RNA. A preparação da biblioteca de mRNA foi feita através do kit de preparação de amostras de mRNA TruSeq Stranded da Illumina. O sequenciamento foi realizado na plataforma HiSeq 2500 (Illumina) através da técnica SBS (Sequenciamento por Síntese) pela empresa MGX - Montpellier Genomix. A análise de expressão diferencial foi realizada a partir das contagens dos reads brutos usando o pacote estatístico DESeq2. O resultado in silico apresentou um total de 17.399 genes diferencialmente expressos entre todos os estresses testados, sendo 2.217, 812, 4.263, 8.008 e 2.099 para o estresse de ABA exógeno, frio, seca, calor e estresse oxidativo, respectivamente. Utilizando esses dados, foram selecionados os genes candidatos mais expressivos nas condições de estresse, os quais foram testadas e seus perfis de expressão diferencial foram confirmados pela técnica de RT-qPCR. Através do estudo in silico deste trabalho foi possível identificar vários genes candidatos respondendo aos diferentes estresses aplicados, o que pode ajudar na compreensão do determinismo genético de tolerância aos estresses abióticos no cafeeiro. A identificação e caracterização desses genes possibilitam a realização de novos estudos da análise da expressão diferencial entre plantas cultivadas no campo e na estufa. Além disso, a prospecção da variabilidade natural utilizando diferentes materiais genéticos pode permitir a identificação e validação de polimorfismos e alelos ligados aos genes, os quais podem ser utilizados para o desenvolvimento de marcadores moleculares associados à tolerância a vários estresses abióticos.

    image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/ Agritroparrow_drop_down
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    Agritrop
    Conference object . 2019
    Data sources: Agritrop
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      image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/ Agritroparrow_drop_down
      image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
      Agritrop
      Conference object . 2019
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  • image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
    Authors: Le Bourgeois, Thomas; Grard, Pierre; Marnotte, Pascal; Rodenburg, Jonne;
    image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/ Agritroparrow_drop_down
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    Agritrop
    Book . 2011
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      image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/ Agritroparrow_drop_down
      image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
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      Book . 2011
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    Authors: Fautrelle, Yves; Allibert, Michel; Etay, Jacqueline; Saadi, B.;

    Colloque avec actes et comité de lecture. Internationale.; International audience; Nous présentons des expériences, utilisant la technique du creuset froid, dans lesquelles des lanthanides, ou d'autres éléments, sont transférés d'une phase saline fluorée vers une phase métallique liquide contenant un agent réducteur tel le Lithium. Du ZrF4 (5 mol%), ou bien du CeF3 (2 mol%), est dissous dans du LiF. Un alliage métallique Sb-Li contenant 5 ou 10 wt % de Lithium, est mis en contact de la phase saline à t = 0. Le taux de transfert est déduit des analyses ICP post mortem effectuées sur des échantillons prélevés dans le métal à pas de temps donnés. La cinétique du transfert est très rapide et dépend de l'agitation électromagnétique.

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    Hyper Article en Ligne; Hal-Diderot
    Other literature type . Conference object . 2007
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      Hyper Article en Ligne; Hal-Diderot
      Other literature type . Conference object . 2007
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    Authors: Houssay-Holzschuch, Myriam; Vacchiani-Marcuzzo, Céline;

    International audience; Territorial recompositions within South African cities bear the influence of global economic forces. The fragmented urban morphology inherited from apartheid composes today with the rapid development of malls. Designed to be cities within the city, or appeal to the exotic imaginary, some offer a new vitality to once decayed environment, and even a haven for desegregation. Other further urban fragmentation while participating in the globalisation of South African cities. Their use of architectural and toponymic references to international and/or vernacular styles can be analysed within the dialectic framework of africanisation/globalisation. We propose to explore these issues through the case study of three Cape Town malls: the Victoria & Albert Waterfront, Century City and Vangate Mall.

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    image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
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    Authors: Martineau, Anne, Martineau;

    Version auteur, plus longue que celle publiée aux Presses de la Sorbonne. Elle est relue et amendée. Dans le texte paru en 2010, un article était signalé en note comme "sous presse". Étant donné qu’il a paru en 2011, nous donnons ici ses références.; International audience; De toutes les versions de la disparition du prophète, la plus terrible est celle du Merlin-Huth (ca 1235-1240) de la Post-Vulgate. La Dame du Lac, ici nommée Niviene, l’y fait mourir de mort lente, en l’enfermant vivant dans un tombeau (et, qui plus est, un tombeau déjà occupé par deux cadavres…) Les critiques modernes s’accordent à y voir une dégradation de l’image de Merlin, parlent de sa « déchéance », de « régression morale », et même de son « retour sous le signe du diable ». Notre article s’inscrit en faux contre ces lectures, et montre qu’au contraire l’auteur (inconnu) de la Suite-Huth a réservé à Merlin une fin terrible, certes, mais pour cela même sublime, et qu’il meurt sauvé.

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    Authors: Toda, Martine;

    International audience; Both frication noise and vowel formants cue the place of articulation of sibilant fricatives (e.g., /s/ and / / in English).However, only few studies have examined the effect of speaker-specific factors. This acoustic study of sibilant fricatives examines how speaker-specific formant information can improve the distinctness of two phonemic categories of sibilants: /s/ vs. / / in French and /s/ vs. /sj/ in Japanese. The results show that the center of gravity of the frication noise, normalized with respect to the subject-specific coefficient of vowel onset or vowel center formants, provides an appreciable improvement in the sibilant distinctness in Japanese. While the distinctness score of the noise is generally higher in French than in Japanese, the F2 onset patterns (/s/

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    Authors: De Méritens, Patrice; Fitoussi, Jean-Paul;
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    Authors: Miras, Claude de;
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    Horizon / Pleins textes
    Other literature type . 1978
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      Horizon / Pleins textes
      Other literature type . 1978
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    Authors: van Chuc Nguyen, ?.; Dandach, A.; Thi Thu Ha Vu, ?.; Fongarland, P.; +1 Authors

    Abstract Tungstated zirconia, ZrW, is reported as an efficient solid acid catalyst in the conversion of cellulose into lactic acid in hydrothermal conditions. The active phase of ZrW is generally ascribed to more or less polymerized WOx domains dispersed on zirconia. In this study, commercial uncalcined and calcined tungstated zirconia were used as catalysts for the conversion of cellulose under hydrothermal conditions at 190 °C. Their activities were also compared with uncalcined zirconium oxyhydroxide, ZrO2-x(OH)2x and calcined zirconia, ZrO2. Contrary to the expected results, it is disclosed that uncalcined ZrW exhibits higher catalytic activity and stability than calcined ZrW. Modifications in the products distribution were observed as glycolic acid formation at the expense of lactic acid formation upon ZrW calcination. Even more surprising, the catalytic activity of W free amorphous zirconium oxyhydroxide ZrO2-x(OH)2x is similar to uncalcined ZrW, with lactic acid as the main product while ZrO2 does not have any catalytic activity. These results suggest that the active phase of these catalysts might combine the couple Zr4+ and OH− species while the role of W species would be of secondary importance.

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    Molecular Catalysis
    Article . 2019
    License: Elsevier TDM
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    Article . 2019
    License: CC BY NC
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      Molecular Catalysis
      Article . 2019
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    Authors: Cécile Mons; Thomas Botzanowski; Anton Nikolaev; Petra Hellwig; +4 Authors

    International audience; Human mitoNEET (mNT) is the first identified Fe−S protein of the mammalian outer mitochondrial membrane. Recently, we demonstrated the involvement of mNT in a specific cytosolic pathway dedicated to the reactivation of oxidatively damaged cytosolic aconitase by cluster transfer. In vitro studies using apo-ferredoxin (FDX) reveal that mNT uses an Fe-based redox switch mechanism to regulate the transfer of its cluster. Using the "gold standard" cluster recipient protein, FDX, we show that this transfer is direct and that only one of the two mNT clusters is transferred when the second one is decomposed. Combining complementary biophysical and biochemical approaches, we show that pH affects both the sensitivity of the cluster to O 2 and dimer stability. Around physiological cytosolic pH, the ability of mNT to transfer its cluster is tightly regulated by the pH. Finally, mNT is extremely resistant to H 2 O 2 compared to ISCU and SufB, two other Fe−S cluster transfer proteins, which is consistent with its involvement in a repair pathway of stress-damaged Fe−S proteins. Taken together, our results suggest that the ability of mNT to transfer its cluster to recipient proteins is not only controlled by the redox state of its cluster but also tightly modulated by the pH of the cytosol. We propose that when pathophysiological conditions such as cancer and neurodegenerative diseases dysregulate cellular pH homeostasis, this pH-dependent regulation of mNT is lost, as is the regulation of cellular pathways under the control of mNT. I ron−sulfur (Fe−S) clusters are evolutionarily ancient and highly conserved prosthetic cofactors. Composed of only iron and sulfur, they are involved in many essential biological processes. 1,2 MitoNEET (mNT), also known as CISD1, is the first identified Fe−S protein of the mammalian outer mitochondrial membrane (OMM). 3,4 This is a small homodimeric protein (13 kDa for each monomer) anchored to the OMM by its 32-amino acid N-terminus with the major part of the protein, including the C-terminal Fe−S binding domain, located in the cytosol. 4 Each monomer accommodates one [2Fe-2S] cluster coordinated by three cysteines (C72, C74, and C83) and one histidine (H87) in a CDGSH domain 5−8 as other members of the NEET protein family, 9 which also includes Miner1 (or CISD2) and Miner2 (or CISD3) in mammals. 10 Although the biological activity of mNT is still debated, 11 studies have shown that it is involved in the regulation of iron/reactive oxygen species homeo-stasis, 12−14 in the regulation of lipid and glucose metabolism , 13,15 and in cell proliferation in breast cancer. 16 In vitro studies revealed that holo-mNT (the form of the protein with the cluster) is able to transfer its Fe−S cluster to very diverse apoprotein (an Fe−S protein, which has lost its cluster) recipients assembling either a [2Fe-2S] cluster as ferredoxin from various organisms, 17,18 human anamorsin 19 and CISD2, 20 or a [4Fe-4S] cluster as mammalian iron regulatory protein-1 (IRP-1)/cytosolic aconitase (c-aconi-tase). 14 On the basis of in cellulo experiments, we showed that mNT is able to repair the oxidatively damaged Fe−S cluster of human IRP-1/c-aconitase by transferring its cluster to the damaged protein. 14 Recently, we started to investigate in depth the in vitro cluster transfer reaction, focusing on the transfer from holo-mNT to [2Fe-2S] recipient protein. We unambiguously demonstrated that oxidized mNT ([2Fe-2S] 2+) triggers cluster transfer, whereas reduction of its cluster abrogates this transfer. Moreover, while O 2 significantly affects the lability of the oxidized mNT cluster, it does not interfere with the cluster

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    Biochemistry
    Article . 2018
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    Authors: Ferreira Torres, Luana; Dechamp, Eveline; Costa Alves, Gabriel Sergio; Marraccini, Pierre; +2 Authors

    O objetivo deste estudo foi obter uma visão geral dos genes ativos em folhas de café arábica variedade Caturra quando submetido a vários estresses abióticos e analisar estes genes identificados in silico por meio da técnica de RT-qPCR. O material vegetal consistiu de plantas in vitro de café arábica expostos por 3 horas sob os diferentes estresses: seca - baixa umidade relativa 9%, frio 5°C, calor 40°C, alta intensidade luminosa 200 μM m-2 s-1 e aplicação exógena de ácido abscísico 10 μM. Após o período de estresse, as folhas de café foram coletadas para a extração de RNA. A preparação da biblioteca de mRNA foi feita através do kit de preparação de amostras de mRNA TruSeq Stranded da Illumina. O sequenciamento foi realizado na plataforma HiSeq 2500 (Illumina) através da técnica SBS (Sequenciamento por Síntese) pela empresa MGX - Montpellier Genomix. A análise de expressão diferencial foi realizada a partir das contagens dos reads brutos usando o pacote estatístico DESeq2. O resultado in silico apresentou um total de 17.399 genes diferencialmente expressos entre todos os estresses testados, sendo 2.217, 812, 4.263, 8.008 e 2.099 para o estresse de ABA exógeno, frio, seca, calor e estresse oxidativo, respectivamente. Utilizando esses dados, foram selecionados os genes candidatos mais expressivos nas condições de estresse, os quais foram testadas e seus perfis de expressão diferencial foram confirmados pela técnica de RT-qPCR. Através do estudo in silico deste trabalho foi possível identificar vários genes candidatos respondendo aos diferentes estresses aplicados, o que pode ajudar na compreensão do determinismo genético de tolerância aos estresses abióticos no cafeeiro. A identificação e caracterização desses genes possibilitam a realização de novos estudos da análise da expressão diferencial entre plantas cultivadas no campo e na estufa. Além disso, a prospecção da variabilidade natural utilizando diferentes materiais genéticos pode permitir a identificação e validação de polimorfismos e alelos ligados aos genes, os quais podem ser utilizados para o desenvolvimento de marcadores moleculares associados à tolerância a vários estresses abióticos.

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    Authors: Le Bourgeois, Thomas; Grard, Pierre; Marnotte, Pascal; Rodenburg, Jonne;
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    Authors: Fautrelle, Yves; Allibert, Michel; Etay, Jacqueline; Saadi, B.;

    Colloque avec actes et comité de lecture. Internationale.; International audience; Nous présentons des expériences, utilisant la technique du creuset froid, dans lesquelles des lanthanides, ou d'autres éléments, sont transférés d'une phase saline fluorée vers une phase métallique liquide contenant un agent réducteur tel le Lithium. Du ZrF4 (5 mol%), ou bien du CeF3 (2 mol%), est dissous dans du LiF. Un alliage métallique Sb-Li contenant 5 ou 10 wt % de Lithium, est mis en contact de la phase saline à t = 0. Le taux de transfert est déduit des analyses ICP post mortem effectuées sur des échantillons prélevés dans le métal à pas de temps donnés. La cinétique du transfert est très rapide et dépend de l'agitation électromagnétique.

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    Other literature type . Conference object . 2007
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      Other literature type . Conference object . 2007
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    Authors: Houssay-Holzschuch, Myriam; Vacchiani-Marcuzzo, Céline;

    International audience; Territorial recompositions within South African cities bear the influence of global economic forces. The fragmented urban morphology inherited from apartheid composes today with the rapid development of malls. Designed to be cities within the city, or appeal to the exotic imaginary, some offer a new vitality to once decayed environment, and even a haven for desegregation. Other further urban fragmentation while participating in the globalisation of South African cities. Their use of architectural and toponymic references to international and/or vernacular styles can be analysed within the dialectic framework of africanisation/globalisation. We propose to explore these issues through the case study of three Cape Town malls: the Victoria & Albert Waterfront, Century City and Vangate Mall.

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