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22 research outcomes, page 1 of 3
  • publication . Article . 2015
    Open Access English
    Authors:
    Cappadocia, Laurent; Pichler, Andrea; Lima, Christopher D.;
    Project: NIH | NE-CAT Center for Advance... (4P41GM103403-14), NIH | Pixel Array Detector for ... (1S10RR029205-01), NIH | Structural Studies of SUM... (5R01GM065872-12), NIH | Macromolecular Crystallog... (3P41GM103473-16S2), NIH | MOUSE GENETICS (2P30CA008748-43), NIH | Macromolecular Crystallog... (3P41RR012408-15S1)

    E3 protein ligases enhance transfer of ubiquitin-like (Ubl) proteins from E2 conjugating enzymes to substrates by stabilizing the thioester-charged E2~Ubl in a closed configuration optimally aligned for nucleophilic attack. Here, we report biochemical and structural dat...

  • publication . Article . Other literature type . 2018
    Open Access
    Authors:
    Aleksandr Milshteyn; Nathaniel R. Braffman; Allison Fay; John B. Biggins; Mirjana Lilic; Sean F. Brady; James Peek; Seth A. Darst; Ian D. Woodworth; Michael S. Glickman; ...
    Publisher: Springer Nature America, Inc
    Project: NIH | Discovery of Antibiotics ... (1R35GM122559-01), NIH | Structure/function analys... (5R01GM114450-02), NIH | NE-CAT Center for Advance... (4P41GM103403-14), NIH | A minimally invasive synt... (1U01GM110714-01A1), NIH | MOUSE GENETICS (2P30CA008748-43), NIH | Structure, function, and ... (5R35GM118130-02), NIH | Crystallization Robot for... (1S10RR027037-01)

    Resistance to rifamycin antibiotics, which target bacterial RNA polymerases, is a growing problem. Here, the authors identify gene clusters from soil metagenomes encoding production of rifamycin analogues that are active against rifampicin-resistant bacteria through a d...

  • publication . Article . 2018
    Open Access English
    Authors:
    Maria Loressa Uson; Ayala Carl; Yehuda Goldgur; Stewart Shuman;
    Publisher: Oxford University Press
    Project: NIH | Pixel Array Detector for ... (1S10RR029205-01), NIH | NE-CAT Center for Advance... (4P41GM103403-14), NIH | MOUSE GENETICS (2P30CA008748-43), NIH | DNA Ligases in Mycobacter... (5R01AI064693-03)

    Abstract Mycobacterium smegmatis FenA is a nucleic acid phosphodiesterase with flap endonuclease and 5′ exonuclease activities. The 1.8 Å crystal structure of FenA reported here highlights as its closest homologs bacterial FEN-family enzymes ExoIX, the Pol1 exonuclease ...

  • publication . Article . 2017
    Open Access English
    Authors:
    John C. Zinder; Mom Das; Christopher D. Lima; Elizabeth V. Wasmuth; Dimitrios Zattas;
    Publisher: eLife Sciences Publications, Ltd
    Project: NIH | Pixel Array Detector for ... (1S10RR029205-01), NIH | NE-CAT Center for Advance... (4P41GM103403-14), NIH | Structural and Functional... (5R01GM079196-07), NIH | MOLECULAR GENETIC ANALYSI... (5F32GM012006-03), NIH | Structural and Biochemica... (5F31GM097910-02), NIH | MOUSE GENETICS (2P30CA008748-43), NIH | Structural studies of RNA... (1R35GM118080-01)

    Nuclear RNA exosomes catalyze a range of RNA processing and decay activities that are coordinated in part by cofactors, including Mpp6, Rrp47, and the Mtr4 RNA helicase. Mpp6 interacts with the nine-subunit exosome core, while Rrp47 stabilizes the exoribonuclease Rrp6 a...

  • publication . Article . 2017
    Open Access English
    Authors:
    Hubin, Elizabeth A; Fay, Allison; Xu, Catherine; Bean, James M; Saecker, Ruth M; Glickman, Michael S; Darst, Seth A; Campbell, Elizabeth A;
    Publisher: eLife Sciences Publications, Ltd
    Project: NIH | NE-CAT Center for Advance... (4P41GM103403-14), NIH | Structure/function analys... (5R01GM114450-02), NIH | Pixel Array Detector for ... (1S10RR029205-01), NIH | MOUSE GENETICS (2P30CA008748-43), NIH | Crystallization Robot for... (1S10RR027037-01)

    RbpA and CarD are essential transcription regulators in mycobacteria. Mechanistic analyses of promoter open complex (RPo) formation establish that RbpA and CarD cooperatively stimulate formation of an intermediate (RP2) leading to RPo; formation of RP2 is likely a bottl...

  • publication . Article . Other literature type . 2019
    Open Access English
    Authors:
    Derek S. Tan; Derek S. Tan; Christopher D. Lima; Christopher D. Lima; Zachary S. Hann; Zachary S. Hann; Xuequan Lu; Michaelyn C. Lux; Michaelyn C. Lux; Shaun K. Olsen; ...
    Publisher: National Academy of Sciences
    Project: NIH | Structural and Functional... (5R01GM079196-07), NIH | Pixel Array Detector for ... (1S10RR029205-01), NIH | Structural Studies of SUM... (5R01GM065872-12), NIH | NE-CAT Center for Advance... (4P41GM103403-14), NIH | Rational Design of Adenyl... (5R01GM100477-08), NIH | MOUSE GENETICS (2P30CA008748-43), NIH | Structural studies of RNA... (1R35GM118080-01), NIH | Tri-Institutional PhD Pro... (1T32GM115327-01)

    Significance Posttranslational protein modification by ubiquitin (Ub) regulates aspects of biology, including protein turnover and the cell cycle. Proteins and enzymes that promote Ub conjugation are therapeutic targets because they are sometimes dysregulated in cancer,...

  • publication . Article . Other literature type . 2017
    Open Access English
    Authors:
    Tasos Gogakos; Tasos Gogakos; J. Fraser Glickman; Antonio Luz; Antonio Luz; Thomas Tuschl; Thomas Tuschl; Jessica Vincent; Rei Okamoto; Jumpei Aida; ...
    Publisher: Nature Publishing Group UK
    Project: NIH | NE-CAT Center for Advance... (4P41GM103403-14), NIH | Chemistry Biology Interfa... (4T32GM065086-14), NIH | Deciphering Post-transcri... (1R35GM119569-01), NIH | MOUSE GENETICS (2P30CA008748-43), NIH | Posttranscriptional regul... (5R01GM104962-02)

    Upon DNA binding cyclic GMP-AMP synthase (cGAS) produces a cyclic dinucleotide, which leads to the upregulation of inflammatory genes. Here the authors develop small molecule cGAS inhibitors, functionally characterize them and present the inhibitor and DNA bound cGAS cr...

  • publication . Article . 2017
    Open Access English
    Authors:
    Yehuda Goldgur; Barbara S. Remus; Stewart Shuman;
    Publisher: Oxford University Press
    Project: NIH | VACCINIA VIRUS MRNA TRANS... (2R01GM042498-10), NIH | Pixel Array Detector for ... (1S10RR029205-01), NIH | NE-CAT Center for Advance... (4P41GM103403-14), NIH | MOUSE GENETICS (2P30CA008748-43)

    Abstract Fungal tRNA ligase (Trl1) is an essential enzyme that repairs RNA breaks with 2′,3′-cyclic-PO4 and 5′-OH ends inflicted during tRNA splicing and non-canonical mRNA splicing in the fungal unfolded protein response. Trl1 is composed of C-terminal cyclic phosphodi...

  • publication . Article . 2018
    Open Access English
    Authors:
    Banerjee, Ankan; Ghosh, Shreya; Goldgur, Yehuda; Shuman, Stewart;
    Publisher: Oxford University Press
    Project: NIH | NE-CAT Center for Advance... (4P41GM103403-14), NIH | Pixel Array Detector for ... (1S10RR029205-01), NIH | MOUSE GENETICS (2P30CA008748-43), NIH | Mechanisms of DNA and RNA... (1R35GM126945-01)

    Abstract Fungal tRNA ligase (Trl1) is an essential enzyme that repairs RNA breaks with 2′,3′-cyclic-PO4 and 5′-OH ends inflicted during tRNA splicing and non-canonical mRNA splicing in the fungal unfolded protein response. Trl1 is composed of C-terminal cyclic phosphodi...

  • publication . Article . 2019
    Open Access English
    Authors:
    Ankan Banerjee; Annum Munir; Leonora Abdullahu; Masad J. Damha; Yehuda Goldgur; Stewart Shuman;
    Publisher: Nature Publishing Group
    Project: NIH | MOUSE GENETICS (2P30CA008748-43), NIH | Pixel Array Detector for ... (1S10RR029205-01), NIH | NE-CAT Center for Advance... (4P41GM103403-14), NIH | New frontiers in extracel... (1R35GM122575-01)

    Tpt1 catalyzes the final essential step in yeast tRNA splicing and is a potential antifungal target. Here the authors provide structural insights into how Tpt1 recognizes a 2’-PO4 RNA splice junction and the mechanism of RNA phospho-ADP-ribosylation.

22 research outcomes, page 1 of 3