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22 research outcomes, page 1 of 3
  • publication . Article . 2015
    Open Access English
    Authors:
    Cappadocia, Laurent; Pichler, Andrea; Lima, Christopher D.;
    Project: NIH | NE-CAT Center for Advance... (4P41GM103403-14), NIH | Pixel Array Detector for ... (1S10RR029205-01), NIH | Structural Studies of SUM... (5R01GM065872-12), NIH | Macromolecular Crystallog... (3P41GM103473-16S2), NIH | MOUSE GENETICS (2P30CA008748-43), NIH | Macromolecular Crystallog... (3P41RR012408-15S1)

    E3 protein ligases enhance transfer of ubiquitin-like (Ubl) proteins from E2 conjugating enzymes to substrates by stabilizing the thioester-charged E2~Ubl in a closed configuration optimally aligned for nucleophilic attack. Here, we report biochemical and structural dat...

  • publication . Article . Other literature type . 2018
    Open Access
    Authors:
    Aleksandr Milshteyn; Nathaniel R. Braffman; Allison Fay; John B. Biggins; Mirjana Lilic; Sean F. Brady; James Peek; Seth A. Darst; Ian D. Woodworth; Michael S. Glickman; ...
    Publisher: Springer Nature America, Inc
    Project: NIH | Discovery of Antibiotics ... (1R35GM122559-01), NIH | Structure/function analys... (5R01GM114450-02), NIH | NE-CAT Center for Advance... (4P41GM103403-14), NIH | A minimally invasive synt... (1U01GM110714-01A1), NIH | MOUSE GENETICS (2P30CA008748-43), NIH | Structure, function, and ... (5R35GM118130-02), NIH | Crystallization Robot for... (1S10RR027037-01)

    Resistance to rifamycin antibiotics, which target bacterial RNA polymerases, is a growing problem. Here, the authors identify gene clusters from soil metagenomes encoding production of rifamycin analogues that are active against rifampicin-resistant bacteria through a d...

  • publication . Article . 2018
    Open Access English
    Authors:
    Maria Loressa Uson; Ayala Carl; Yehuda Goldgur; Stewart Shuman;
    Publisher: Oxford University Press
    Project: NIH | Pixel Array Detector for ... (1S10RR029205-01), NIH | NE-CAT Center for Advance... (4P41GM103403-14), NIH | MOUSE GENETICS (2P30CA008748-43), NIH | DNA Ligases in Mycobacter... (5R01AI064693-03)

    Abstract Mycobacterium smegmatis FenA is a nucleic acid phosphodiesterase with flap endonuclease and 5′ exonuclease activities. The 1.8 Å crystal structure of FenA reported here highlights as its closest homologs bacterial FEN-family enzymes ExoIX, the Pol1 exonuclease ...

  • publication . Article . 2017
    Open Access English
    Authors:
    John C. Zinder; Mom Das; Christopher D. Lima; Elizabeth V. Wasmuth; Dimitrios Zattas;
    Publisher: eLife Sciences Publications, Ltd
    Project: NIH | Pixel Array Detector for ... (1S10RR029205-01), NIH | NE-CAT Center for Advance... (4P41GM103403-14), NIH | Structural and Functional... (5R01GM079196-07), NIH | MOLECULAR GENETIC ANALYSI... (5F32GM012006-03), NIH | Structural and Biochemica... (5F31GM097910-02), NIH | MOUSE GENETICS (2P30CA008748-43), NIH | Structural studies of RNA... (1R35GM118080-01)

    Nuclear RNA exosomes catalyze a range of RNA processing and decay activities that are coordinated in part by cofactors, including Mpp6, Rrp47, and the Mtr4 RNA helicase. Mpp6 interacts with the nine-subunit exosome core, while Rrp47 stabilizes the exoribonuclease Rrp6 a...

  • publication . Article . 2017
    Open Access English
    Authors:
    Hubin, Elizabeth A; Fay, Allison; Xu, Catherine; Bean, James M; Saecker, Ruth M; Glickman, Michael S; Darst, Seth A; Campbell, Elizabeth A;
    Publisher: eLife Sciences Publications, Ltd
    Project: NIH | NE-CAT Center for Advance... (4P41GM103403-14), NIH | Structure/function analys... (5R01GM114450-02), NIH | Pixel Array Detector for ... (1S10RR029205-01), NIH | MOUSE GENETICS (2P30CA008748-43), NIH | Crystallization Robot for... (1S10RR027037-01)

    RbpA and CarD are essential transcription regulators in mycobacteria. Mechanistic analyses of promoter open complex (RPo) formation establish that RbpA and CarD cooperatively stimulate formation of an intermediate (RP2) leading to RPo; formation of RP2 is likely a bottl...

  • publication . Article . Other literature type . 2019
    Open Access English
    Authors:
    Derek S. Tan; Derek S. Tan; Christopher D. Lima; Christopher D. Lima; Zachary S. Hann; Zachary S. Hann; Xuequan Lu; Michaelyn C. Lux; Michaelyn C. Lux; Shaun K. Olsen; ...
    Publisher: National Academy of Sciences
    Project: NIH | Structural and Functional... (5R01GM079196-07), NIH | Pixel Array Detector for ... (1S10RR029205-01), NIH | Structural Studies of SUM... (5R01GM065872-12), NIH | NE-CAT Center for Advance... (4P41GM103403-14), NIH | Rational Design of Adenyl... (5R01GM100477-08), NIH | MOUSE GENETICS (2P30CA008748-43), NIH | Structural studies of RNA... (1R35GM118080-01), NIH | Tri-Institutional PhD Pro... (1T32GM115327-01)

    Significance Posttranslational protein modification by ubiquitin (Ub) regulates aspects of biology, including protein turnover and the cell cycle. Proteins and enzymes that promote Ub conjugation are therapeutic targets because they are sometimes dysregulated in cancer,...

  • publication . Article . Other literature type . 2017
    Open Access
    Authors:
    Hongda Huang; Hongda Huang; Aayushi Y Jain; Dinshaw J. Patel; Dominik Hoelper; Peter W. Lewis;
    Publisher: Springer Nature
    Project: NIH | NE-CAT Center for Advance... (4P41GM103403-14), NIH | MOUSE GENETICS (2P30CA008748-43)

    The ATRX-DAXX histone chaperone complex incorporates H3.3 in heterochromatin in a replication-independent manner. Here, the authors present a high-resolution x-ray crystal structure of an interaction surface between ATRX and DAXX, and characterize ATRX-dependent and-ind...

  • publication . Article . 2020
    Open Access English
    Authors:
    Shreya Ghosh; Yehuda Goldgur; Stewart Shuman;
    Publisher: Oxford University Press
    Project: NIH | Pixel Array Detector for ... (1S10RR029205-01), NIH | NE-CAT Center for Advance... (4P41GM103403-14), NIH | MOUSE GENETICS (2P30CA008748-43), NIH | Mechanisms of DNA and RNA... (1R35GM126945-01), NIH | DNA Ligases in Mycobacter... (5R01AI064693-03)

    <jats:title>Abstract</jats:title> <jats:p>Mycobacterial Pol1 is a bifunctional enzyme composed of an N-terminal DNA flap endonuclease/5′ exonuclease domain (FEN/EXO) and a C-terminal DNA polymerase domain (POL). Here we document additional functions of Pol1: FEN activit...

  • publication . Article . Other literature type . 2018
    Open Access
    Authors:
    Akiko Koide; Stephen B. Long; Shohei Koide; Leanne Pedi; Melinda M. Diver;
    Publisher: Springer Science and Business Media LLC
    Project: NIH | NE-CAT Center for Advance... (4P41GM103403-14), NIH | Pixel Array Detector for ... (1S10RR029205-01), NIH | Bridge 1: Conformational ... (2U54GM087519-06), NIH | MOLECULAR GENETIC ANALYSI... (5F32GM012006-03), NIH | MOUSE GENETICS (2P30CA008748-43)

    The X-ray structure of the integral membrane protein isoprenylcysteine carboxyl methyltransferase suggests mechanisms by which it recognizes both water-soluble and membrane-bound reactants to catalyze the methylation of RAS and other CAAX proteins at the membrane-cytoso...

  • publication . Article . 2019
    Open Access English
    Authors:
    Hann, Zachary S.; Metzger, Meredith B.; Weissman, Allan M.; Lima, Christopher D.;
    Publisher: International Union of Crystallography
    Project: NIH | Pixel Array Detector for ... (1S10RR029205-01), NIH | Structural and Functional... (5R01GM079196-07), NIH | NE-CAT Center for Advance... (4P41GM103403-14), NIH | MOUSE GENETICS (2P30CA008748-43), NIH | Structural studies of RNA... (1R35GM118080-01)

    A 1.7 Å resolution co-crystal structure of U7BR from Schizosaccharomyces pombe in complex with Ubc7 is reported. Structural comparisons and biochemistry suggest that U7BR presents a steric impediment to E1 binding and inhibits E1-mediated charging, respectively.

22 research outcomes, page 1 of 3