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19 research outcomes, page 1 of 2
  • publication . Article . 2017
    Open Access English
    Authors:
    Elizabeth V Wasmuth; John C Zinder; Dimitrios Zattas; Mom Das; Christopher D Lima;
    Publisher: eLife Sciences Publications, Ltd
    Project: NIH | MOLECULAR GENETIC ANALYSI... (5F32GM012006-03), NIH | MOUSE GENETICS (2P30CA008748-43), NIH | Structural studies of RNA... (1R35GM118080-01), NIH | NE-CAT Center for Advance... (4P41GM103403-14), NIH | Pixel Array Detector for ... (1S10RR029205-01), NIH | Structural and Functional... (5R01GM079196-07), NIH | Structural and Biochemica... (5F31GM097910-02)

    Nuclear RNA exosomes catalyze a range of RNA processing and decay activities that are coordinated in part by cofactors, including Mpp6, Rrp47, and the Mtr4 RNA helicase. Mpp6 interacts with the nine-subunit exosome core, while Rrp47 stabilizes the exoribonuclease Rrp6 a...

  • publication . Other literature type . Article . 2018
    Open Access
    Authors:
    James Peek; Mirjana Lilic; Daniel Montiel; Aleksandr Milshteyn; Ian Woodworth; John B. Biggins; Melinda A. Ternei; Paula Y. Calle; Michael Danziger; Thulasi Warrier; ...
    Publisher: Springer Science and Business Media LLC
    Project: NIH | A minimally invasive synt... (1U01GM110714-01A1), NIH | NE-CAT Center for Advance... (4P41GM103403-14), NIH | MOUSE GENETICS (2P30CA008748-43), NIH | Crystallization Robot for... (1S10RR027037-01), NIH | Structure/function analys... (5R01GM114450-02), NIH | Discovery of Antibiotics ... (1R35GM122559-01), NIH | Structure, function, and ... (5R35GM118130-02)

    Resistance to rifamycin antibiotics, which target bacterial RNA polymerases, is a growing problem. Here, the authors identify gene clusters from soil metagenomes encoding production of rifamycin analogues that are active against rifampicin-resistant bacteria through a d...

  • publication . Article . 2018
    Open Access English
    Authors:
    Maria Loressa Uson; Ayala Carl; Yehuda Goldgur; Stewart Shuman;
    Publisher: Oxford University Press
    Project: NIH | DNA Ligases in Mycobacter... (5R01AI064693-03), NIH | NE-CAT Center for Advance... (4P41GM103403-14), NIH | MOUSE GENETICS (2P30CA008748-43), NIH | Pixel Array Detector for ... (1S10RR029205-01)

    Abstract Mycobacterium smegmatis FenA is a nucleic acid phosphodiesterase with flap endonuclease and 5′ exonuclease activities. The 1.8 Å crystal structure of FenA reported here highlights as its closest homologs bacterial FEN-family enzymes ExoIX, the Pol1 exonuclease ...

  • publication . Other literature type . Article . 2019
    Open Access
    Authors:
    Christopher Lima; Shaun Olsen; Michaelyn C. Lux; Zachary Hann;
    Publisher: Proceedings of the National Academy of Sciences
    Project: NIH | NE-CAT Center for Advance... (4P41GM103403-14), NIH | Rational Design of Adenyl... (5R01GM100477-08), NIH | MOUSE GENETICS (2P30CA008748-43), NIH | Pixel Array Detector for ... (1S10RR029205-01), NIH | Structural studies of RNA... (1R35GM118080-01), NIH | Tri-Institutional PhD Pro... (1T32GM115327-01), NIH | Structural and Functional... (5R01GM079196-07), NIH | Structural Studies of SUM... (5R01GM065872-12)

    Significance Posttranslational protein modification by ubiquitin (Ub) regulates aspects of biology, including protein turnover and the cell cycle. Proteins and enzymes that promote Ub conjugation are therapeutic targets because they are sometimes dysregulated in cancer,...

  • publication . Article . 2017
    Open Access
    Authors:
    Remus, Barbara S.; Goldgur, Yehuda; Shuman, Stewart;
    Publisher: Oxford University Press (OUP)
    Project: NIH | MOUSE GENETICS (2P30CA008748-43), NIH | Pixel Array Detector for ... (1S10RR029205-01), NIH | VACCINIA VIRUS MRNA TRANS... (2R01GM042498-10), NIH | NE-CAT Center for Advance... (4P41GM103403-14)

    Abstract Fungal tRNA ligase (Trl1) is an essential enzyme that repairs RNA breaks with 2′,3′-cyclic-PO4 and 5′-OH ends inflicted during tRNA splicing and non-canonical mRNA splicing in the fungal unfolded protein response. Trl1 is composed of C-terminal cyclic phosphodi...

  • publication . Article . Other literature type . 2017
    Open Access
    Authors:
    Jessica Vincent; Carolina Adura; Pu Gao; Antonio Luz; Lodoe Lama; Yasutomi Asano; Rei Okamoto; Toshihiro Imaeda; Jumpei Aida; Katherine Rothamel; ...
    Publisher: Springer Science and Business Media LLC
    Project: NIH | Posttranscriptional regul... (5R01GM104962-02), NIH | MOUSE GENETICS (2P30CA008748-43), NIH | Chemistry Biology Interfa... (4T32GM065086-14), NIH | Deciphering Post-transcri... (1R35GM119569-01), NIH | NE-CAT Center for Advance... (4P41GM103403-14)

    Upon DNA binding cyclic GMP-AMP synthase (cGAS) produces a cyclic dinucleotide, which leads to the upregulation of inflammatory genes. Here the authors develop small molecule cGAS inhibitors, functionally characterize them and present the inhibitor and DNA bound cGAS cr...

  • publication . Article . Other literature type . 2019
    Open Access English
    Authors:
    Hann, Zachary S.; Metzger, Meredith B.; Weissman, Allan M.; Lima, Christopher D.;
    Publisher: International Union of Crystallography
    Project: NIH | Structural and Functional... (5R01GM079196-07), NIH | MOUSE GENETICS (2P30CA008748-43), NIH | Structural studies of RNA... (1R35GM118080-01), NIH | Pixel Array Detector for ... (1S10RR029205-01), NIH | NE-CAT Center for Advance... (4P41GM103403-14)

    A 1.7 Å resolution co-crystal structure of U7BR from Schizosaccharomyces pombe in complex with Ubc7 is reported. Structural comparisons and biochemistry suggest that U7BR presents a steric impediment to E1 binding and inhibits E1-mediated charging, respectively.

  • publication . Article . 2017
    Open Access
    Authors:
    Wenfu Ma; Elena Goldberg; Jonathan Goldberg;
    Publisher: eLife Sciences Publications, Ltd
    Project: NIH | Pixel Array Detector for ... (1S10RR029205-01), NIH | NE-CAT Center for Advance... (4P41GM103403-14), NIH | MOLECULAR GENETIC ANALYSI... (5F32GM012006-03), NIH | MOUSE GENETICS (2P30CA008748-43)

    Native cargo proteins exit the endoplasmic reticulum (ER) in COPII-coated vesicles, whereas resident and misfolded proteins are substantially excluded from vesicles by a retention mechanism that remains unresolved. We probed the ER retention process using the proteostas...

  • publication . Article . 2019
    Open Access English
    Authors:
    Ankan Banerjee; Annum Munir; Leonora Abdullahu; Masad J. Damha; Yehuda Goldgur; Stewart Shuman;
    Publisher: Nature Publishing Group UK
    Project: NIH | NE-CAT Center for Advance... (4P41GM103403-14), NIH | MOUSE GENETICS (2P30CA008748-43), NIH | Pixel Array Detector for ... (1S10RR029205-01), NIH | New frontiers in extracel... (1R35GM122575-01)

    Tpt1 catalyzes the final essential step in yeast tRNA splicing and is a potential antifungal target. Here the authors provide structural insights into how Tpt1 recognizes a 2’-PO4 RNA splice junction and the mechanism of RNA phospho-ADP-ribosylation.

  • publication . Article . Other literature type . 2016
    Open Access English
    Authors:
    Christopher Lima; Frederick C. Streich Jr.;
    Project: NIH | Structural Studies of SUM... (5R01GM065872-12), NIH | NE-CAT Center for Advance... (4P41GM103403-14), NIH | MOUSE GENETICS (2P30CA008748-43), NIH | Structural studies of RNA... (1R35GM118080-01), NIH | Pixel Array Detector for ... (1S10RR029205-01)

    Post-translational protein modification by ubiquitin (Ub) and ubiquitin-like (Ubl) proteins such as small ubiquitin-like modifier (SUMO) regulates processes including protein homeostasis, the DNA damage response, and the cell cycle. Proliferating cell nuclear antigen (P...

19 research outcomes, page 1 of 2
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