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13 research outcomes, page 1 of 2
  • publication . Other literature type . Preprint . 2018
    Open Access
    Authors:
    Murakami, Hajime; Lam, Isabel; Song, Jacquelyn; van Overbeek, Megan; Keeney, Scott;
    Publisher: bioRxiv
    Project: NIH | MOUSE GENETICS (2P30CA008748-43), NIH | Functional Characterizati... (5F31GM097861-02), NIH | Mechanism of meiotic reco... (5R01GM058673-15), NIH | Mechanism and regulation ... (5R35GM118092-03), NIH | The Role of Chromatin Arc... (5F32GM096692-02)

    <jats:p>To segregate accurately during meiosis, homologous chromosomes in most species must recombine. Very small chromosomes would risk missegregation if recombination were randomly distributed, so the double-strand breaks (DSBs) that initiate recombination are not hap...

  • publication . Other literature type . Preprint . Article . 2019
    Open Access
    Authors:
    Yamada, Shintaro; Hinch, Anjali Gupta; Kamido, Hisashi; Zhang, Yongwei; Edelmann, Winfried; Keeney, Scott;
    Publisher: bioRxiv
    Project: NIH | MOUSE GENETICS (2P30CA008748-43), NIH | Mechanism and regulation ... (5R35GM118092-03)

    <jats:p>Exonucleolytic resection, critical to repair double-strand breaks (DSBs) by recombination, is not well understood, particularly in mammalian meiosis. Here, we define structures of resected DSBs in mouse spermatocytes genome-wide at nucleotide resolution. Resecti...

  • publication . Preprint . Other literature type . Article . 2017
    Open Access English
    Authors:
    Shintaro Yamada; Seoyoung Kim; Sam E. Tischfield; Maria Jasin; Julian Lange; Scott Keeney;
    Publisher: Cold Spring Harbor Laboratory
    Project: NIH | MOUSE GENETICS (2P30CA008748-43), NIH | Role of Spo11 and recombi... (9R01GM105421-11A1), NIH | Mechanism and regulation ... (5R35GM118092-03), NIH | Fluorescence microscopy f... (3R35GM118175-01S1)

    <jats:title>Abstract</jats:title><jats:p>The SPO11-generated DNA double-strand breaks (DSBs) that initiate meiotic recombination occur non-randomly across genomes, but mechanisms shaping their distribution and repair remain incompletely understood. Here, we expand on re...

  • publication . Article . Other literature type . 2018
    Open Access
    Authors:
    Carla M. Abreu; Rohit Prakash; Peter J. Romanienko; Ignasi Roig; Scott Keeney; Maria Jasin;
    Project: NIH | Deciphering the epistatic... (5F32GM110978-02), NIH | MOUSE GENETICS (2P30CA008748-43), NIH | Fluorescence microscopy f... (3R35GM118175-01S1), NIH | Mechanism and regulation ... (5R35GM118092-03), NIH | HOMOLOGY-DIRECTED DNA REP... (5R01CA185660-04)

    Homologous recombination ensures genome integrity during meiotic recombination. Here the authors reveal that factors SWS1 and SWSAP1 are critical for meiotic homologues recombination, particularly in promoting assembly of RAD51 and DMC1 on early recombination intermedia...

  • publication . Preprint . Other literature type . 2018
    Open Access
    Authors:
    Boekhout, Michiel; Karasu, Mehmet E.; Wang, Juncheng; Acquaviva, Laurent; Pratto, Florencia; Brick, Kevin; Eng, Diana Y.; Camerini-Otero, R. Daniel; Patel, Dinshaw J.; Keeney, Scott;
    Publisher: Cold Spring Harbor Laboratory
    Project: NIH | MOUSE GENETICS (2P30CA008748-43), NIH | Pixel Array Detector for ... (1S10RR029205-01), NIH | Mechanism and regulation ... (5R35GM118092-03)

    <jats:p>Double-strand breaks (DSBs) initiate the homologous recombination that is crucial for meiotic chromosome pairing and segregation. Here we unveil mouse ANKRD31 as a lynchpin governing multiple aspects of DSB formation. Spermatocytes lacking ANKRD31 have altered D...

  • publication . Preprint . 2020
    Open Access
    Authors:
    Bouuaert, Corentin Claeys; Tischfield, Sam E.; Pu, Stephen; Mimitou, Eleni P.; Arias-Palomo, Ernesto; Berger, James M.; Keeney, Scott;
    Publisher: Cold Spring Harbor Laboratory
    Project: NIH | MOUSE GENETICS (2P30CA008748-43), NIH | Mechanism and regulation ... (5R35GM118092-03), NIH | Asymmetric flow FFF and C... (1S10RR023748-01)

    <jats:p>Spo11, which makes DNA double-strand breaks (DSBs) essential for meiotic recombination, is poorly understood mechanistically because it has been recalcitrant to biochemical study. Here, we provide a molecular analysis of <jats:italic>S. cerevisiae</jats:italic> ...

  • publication . Article . Preprint . Other literature type . 2016
    Open Access
    Authors:
    Mimitou, Eleni P.; Yamada, Shintaro; Keeney, Scott;
    Publisher: American Association for the Advancement of Science (AAAS)
    Project: NIH | MOUSE GENETICS (2P30CA008748-43), NIH | Mechanism of meiotic reco... (5R01GM058673-15), NIH | Mechanism and regulation ... (5R35GM118092-03)

    <jats:title>Abstract</jats:title><jats:p>The DNA double-strand breaks that initiate homologous recombination during meiosis are subject to extensive 5′→3′ exonucleolytic processing. This resection is a central and conserved feature of recombination, yet its mechanism is...

  • publication . Preprint . Part of book or chapter of book . Other literature type . 2017
    Open Access
    Authors:
    Mimitou, Eleni P.; Keeney, Scott;
    Publisher: Cold Spring Harbor Laboratory
    Project: NIH | Mechanism of meiotic reco... (5R01GM058673-15), NIH | MOUSE GENETICS (2P30CA008748-43), NIH | Mechanism and regulation ... (5R35GM118092-03)

    <jats:title>Abstract</jats:title><jats:p>During meiosis, the specialized cell division giving rise to gametes, numerous DNA doublestrand breaks (DSBs) are introduced at multiple places throughout the genome by the topoisomerase-like protein Spo11. Homologous recombinati...

  • publication . Preprint . 2020
    Open Access English
    Authors:
    Xiaojing Mu; Hajime Murakami; Neeman Mohibullah; Scott Keeney;
    Publisher: Cold Spring Harbor Laboratory
    Project: NIH | MOUSE GENETICS (2P30CA008748-43), NIH | Mechanism and regulation ... (5R35GM118092-03)

    <jats:p>The number of DNA double-strand breaks (DSBs) initiating meiotic recombination is elevated in <jats:italic>Saccharomyces cerevisiae</jats:italic> mutants that are globally defective in forming crossovers and synaptonemal complex (SC), a protein scaffold juxtapos...

  • publication . Preprint . Other literature type . Article . 2017
    Open Access English
    Authors:
    Agnieszka Lukaszewicz; Julian Lange; Scott Keeney; Maria Jasin;
    Publisher: Cold Spring Harbor Laboratory
    Project: NIH | Fluorescence microscopy f... (3R35GM118175-01S1), NIH | MOUSE GENETICS (2P30CA008748-43), NIH | Mechanism and regulation ... (5R35GM118092-03)

    <jats:title>ABSTRACT</jats:title><jats:p>DNA double-strand breaks (DSBs) generated by the SPO11 protein initiate meiotic recombination, an essential process for successful chromosome segregation during gametogenesis. The activity of SPO11 is controlled by multiple facto...

13 research outcomes, page 1 of 2
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