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Genome-wide screens are a powerful technique to dissect the complex network of genes regulating diverse cellular phenotypes. The recent adaptation of the CRISPR-Cas9 system for genome engineering has revolutionized functional genomic screening. Here, we present protocols used to introduce Cas9 into human lymphoma cell lines, produce high-titer lentivirus of a genome-wide sgRNA library, transduce and culture cells during the screen, isolate genomic DNA, and prepare a custom library for next-generation sequencing. These protocols were tailored for loss-of-function CRISPR screens in human lymphoma cell lines but are highly amenable for other experimental purposes.

[p. 445] THE SUBJECT OF this paper is a collection of essays composed, in the words of the title "as the brush goes," by Sŏp'o, that is, by Kim Manjung (1637-1692). He is well known, as the author of a masterpiece of Korean fiction, the novel Nine Cloud Dream (Kuunmong). He is also credited with another novel, less famous but not less interesting, The Record of a Southern Journey (Namjŏng ki), which is especially well documented and on which I have already published several articles.

International audience; Orchestras of Digital Musical Instruments (DMIs) enable new musical collaboration possibilities, extending those of acoustic and electric orchestras. However the creation and development of these orchestras remain constrained. In fact, each new musical collaboration system or orchestra piece relies on a fixed number of musicians, a fixed set of instruments (often only one), and a fixed subset of possible modes of collaboration. In this paper, we describe a unified framework that enables the design of Digital Orchestras with potentially different DMIs and an expand-able set of collaboration modes. It relies on research done on analysis and classification of traditional and digital orchestras, on research in Collaborative Virtual Environments, and on interviews of musicians and composers. The BOEUF framework consists of a classification of modes of collaboration and a set of components for modelling digital orchestras. Integrating this framework into DMIs will enable advanced musical collaboration modes to be used in any digital orchestra, including spontaneous jam sessions.

Many data management applications, such as setting up Web portals, managing enterprise data, managing community data, and sharing scientific data, require integrating data from multiple sources. Each of these sources provides a set of values and different sources can often provide conflicting values. To present quality data to users, it is critical to resolve conflicts and discover values that reflect the real world; this task is called {\em data fusion}. This paper describes a novel approach that finds true values from conflicting information when there are a large number of sources, among which some may copy from others. We present a case study on real-world data showing that the described algorithm can significantly improve accuracy of truth discovery and is scalable when there are a large number of data sources. WAIM 2013

International audience; This chapter introduces several state of the art techniques that could help to make deep underwater archaeological photogrammetric surveys easier, faster, more accurate, and to provide more visually appealing representations in 2D and 3D for both experts and public. We detail how the 3D captured data is analysed and then represented using ontologies, and how this facilitates interdisciplinary interpretation and cooperation. Towards more automation, we present a new method that adopts a deep learning approach for the detection and the recognition of objects of interest, amphorae for example. In order to provide more readable, direct and clearer illustrations, we describe several techniques that generate different styles of sketches out of orthophotos developed using neural networks. In the same direction, we present the Non-Photorealistic Rendering (NPR) technique, which converts a 3D model into a more readable 2D representation that is more useful to communicate and simplifies the identification of objects of interest. Regarding public dissemination, we demonstrate how recent advances in virtual reality to provide an accurate, high resolution, amusing and appropriate visualization tool that offers the public the possibility to ‘visit’ an unreachable archaeological site. Finally, we conclude by introducing the plenoptic approach, a new promising technology that can change the future of the photogrammetry by making it easier and less time consuming and that allows a user to create a 3D model using only one camera shot. Here, we introduce the concepts, the developing process, and some results, which we obtained with underwater imaging.

International audience; The relative efficiency of auctions and negotiations is still a puzzle in the literature. While auctions are the prescribed procedures and the most used ones for public procurement, in the private sector, where buyers are free to choose their purchasing method, competitive tendering is far from being their preferred option (Bajari et al. 2009). In addition, recent empirical studies (Estache et al. 2009, Bajari et al. 2009) highlight some failures of auction procedures and identify conditions under which negotiation is more efficient. In particular, they show that auctions perform poorly when projects are complex. In this paper, our aim is to contribute to this debate by providing an empirical analysis of how awarding mechanisms are chosen in public procurement in France. To this end, we examine a comprehensive database of 76,188 observations corresponding to the entire set of public procurement work contracts awarded between 2005 and 2007 in the construction sector. We find empirical regularities regarding the choice of awarding procedures by public buyers. However, most of these regularities do not coincide with what the theoretical literature considers as transaction-cost minimizing behaviours. In particular, the size of the construction projects as well as the length of contracts do not appear as key determinants of the choice of awarding procedures, which translates into costly renegotiations.

This chapter examines the interactive communications of geographically distant virtual classrooms, connected via virtual aids ranging from e-mails to videoconferences. The combination is crucial: through diverse filters, virtual teams mediate a final text for a new language and culture. The authors use linguistic pragmatics as a mechanism to analyze and assess the efficiency and the meaningfulness of such communications. They then use this approach to recommend best practices for educators teaching in cross-cultural virtual environments.

National audience; This chapter focuses on the economic mechanisms at work in recent models of advertising finance in media markets developed around the concept of two-sided markets. The objective is to highlight new and original insights from this approach, and to clarify the conceptual aspects. The chapter first develops a canonical model of two-sided markets for advertising, where platforms deliver content to consumers and resell their “attention” to advertisers. A key distinction is drawn between free media and pay media, where the former result from the combination of valuable consumer attention and low ad-nuisance cost. The first part discusses various conceptual issues such as equilibrium concepts and the nature of inefficiencies in advertising markets, and concrete issues such as congestion and second-degree discrimination. The second part is devoted to recent contributions on issues arising when consumers patronize multiple platforms. In this case, platforms can only charge incremental values to advertisers, which reduce their market power and affect their price strategies and advertising levels. The last part discusses the implications of the two-sided nature of the media markets for the choice of content and diversity.

International audience; The study area mainly includes the continental shelves of two European states (France and Spain), three North African countries (Algeria, Morocco and Tunisia), and Gibraltar and Monaco. Overall, the total shelf area in the western Mediterranean down to the 150 m isobath is 208,000 km2 within a total basin area of 1,343,620 km2. There is a major difference in the degree of available information from EU member states and North African countries.