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247 research outcomes, page 1 of 25
  • publication . Article . Other literature type . 2018
    Open Access English
    Authors:
    Michael Groll; Henry Nguyen; Sreekumar Vellalath; Daniel Romo;
    Publisher: MDPI AG
    Project: NIH | Synthetic/Mechanistic Stu... (5R37GM052964-22), EC | BIOSTRUCT-X (283570)

    Upon acylation of the proteasome by the β-lactone inhibitor salinosporamide A (SalA), tetrahydrofuran formation occurs by intramolecular alkylation of the incipient alkoxide onto the choroethyl sidechain and irreversibly blocks the active site. Our previously descr...

  • publication . Article . Other literature type . 2015
    Open Access
    Authors:
    Gruszka, Dominika T.; Whelan, Fiona; Farrance, Oliver E.; Fung, Herman K. H.; Paci, Emanuele; Jeffries, Cy M.; Svergun, Dmitri I.; Baldock, Clair; Baumann, Christoph G.; Brockwell, David J.; ...
    Publisher: Springer Science and Business Media LLC
    Project: RCUK | Structure and folding of ... (BB/J005029/1), WT | Multidisciplinary studies... (095195), RCUK | Investigating E. coli cel... (BB/G019452/1), RCUK | Investigating E. coli cel... (BB/G020671/1), EC | BIOSTRUCT-X (283570), RCUK | Structure and Folding of ... (BB/J006459/1)

    Staphylococcal biofilm formation is promoted by the surface protein SasG. Here, the authors characterize the structure and remarkable mechanical strength of the repeat region of SasG, and show how elongation is achieved by obligate folding of the disordered regions with...

  • publication . Article . 2017
    Open Access English
    Authors:
    Leticia Monjas; Lotteke J. Y. M. Swier; Inda Setyawati; Dirk J. Slotboom; Anna K. H. Hirsch;
    Project: NWO | Bacterial vitamin uptake (2300169733), EC | ABCTRANSPORT (282083), NWO | Small molecule modulators... (2300170634), EC | BIOSTRUCT-X (283570), NWO | Research Centre for Funct... (2300176518)

    We applied dynamic combinatorial chemistry (DCC) to identify ligands of ThiT, the S-component of the energy-coupling factor (ECF) transporter for thiamine in Lactococcus lactis. We used a pre-equilibrated dynamic combinatorial library (DCL) and saturation-transfer diffe...

  • publication . Article . 2018
    Open Access English
    Authors:
    Julia J. Griese; Rui M. M. Branca; Vivek Srinivas; Martin Högbom;
    Publisher: Springer Berlin Heidelberg
    Project: EC | BIOSTRUCT-X (283570), EC | HIGH-GEAR (724394)

    R2-like ligand-binding oxidases contain a dinuclear metal cofactor which can consist either of two iron ions or one manganese and one iron ion, but the heterodinuclear Mn/Fe cofactor is the preferred assembly in the presence of MnII and FeII in vitro. We have previously...

  • publication . Article . 2013
    Open Access English
    Authors:
    Angelucci, F.; Saccoccia, F.; Ardini, M.; Boumis, G.; Brunori, M.; Di Leandro, L.; Ippolitl, R.; Miele, A. E.; Natoli, G.; Scotti, S.; ...
    Publisher: HAL CCSD
    Project: EC | BIOSTRUCT-X (283570)

    Members of the typical 2-Cys peroxiredoxin (Prx) subfamily represent an intriguing example of protein moonlighting behavior since this enzyme shifts function: indeed, upon chemical stimuli, such as oxidative stress, Prx undergoes a switch from peroxidase to molecular ch...

  • publication . Article . 2015
    Open Access English
    Authors:
    Hassan Noor; Kori Lokesh D.; Gandini Rosaria; Patel Bharat K. C.; Divne Christina; Tan Tien Chye;
    Publisher: International Union of Crystallography
    Project: EC | BIOSTRUCT-X (283570)

    A gene from the heterotrophic, halothermophilic marine bacterium Halothermothrix orenii has been cloned and overexpressed in Escherichia coli. This gene encodes the only glycoside hydrolase of family 43 (GH43) produced by H. orenii. The crystal structure of the H. oreni...

  • publication . Preprint . Other literature type . Article . 2019
    Open Access English
    Authors:
    Hay, Iain M.; Fearnley, Gareth W.; Rios, Pablo; Köhn, Maja; Sharpe, Hayley J.; Deane, Janet E.;
    Publisher: Cold Spring Harbor Laboratory
    Project: WT , EC | BIOSTRUCT-X (283570)

    Receptor-linked protein tyrosine phosphatases (RPTPs) usually contain an active membrane proximal domain and an inactive pseudophosphatase domain. Here, the authors characterize an RPTP with two pseudophosphatase domains, providing evidence that it may act as a decoy re...

  • publication . Article . Other literature type . 2018
    Open Access
    Authors:
    Czyrko, Justyna; Sliwiak, Joanna; Imiolczyk, Barbara; Gdaniec, Zofia; Jaskolski, Mariusz; Brzezinski, Krzysztof;
    Publisher: Springer Science and Business Media LLC
    Project: EC | BIOSTRUCT-X (283570)

    Abstract S-adenosyl-l-homocysteine hydrolase from Pseudomonas aeruginosa (PaSAHase) coordinates one K+ ion and one Zn2+ ion in the substrate binding area. The cations affect the enzymatic activity and substrate binding but the molecular mechanisms of their action are un...

  • publication . Article . 2020
    Open Access English
    Authors:
    García-García, Ana; Ceballos-Laita, Laura; Serna, Sonia; Artschwager, Raik; Reichardt, Niels C.; Corzana, Francisco; Hurtado-Guerrero, Ramón;
    Project: EC | BIOSTRUCT-X (283570)

    Core-fucosylation is an essential biological modification by which a fucose is transferred from GDP-ß-L-fucose to the innermost N-acetylglucosamine residue of N-linked glycans. A single human enzyme a1,6-fucosyltransferase (FUT8) is the only enzyme responsible for this ...

  • publication . Article . Other literature type . 2014
    Open Access English
    Authors:
    Schulte, Tim; Lofling, Jonas; Mikaelsson, Cecilia; Kikhney, Alexey; Hentrich, Karina; Diamante, Aurora; Ebel, Christine; Normark, Staffan; Svergun, Dmitri; Henriques-Normark, Birgitta; ...
    Publisher: HAL CCSD
    Project: EC | BIOSTRUCT-X (283570)

    International audience; Streptococcus pneumoniae is a major human pathogen, and a leading cause of disease and death worldwide. Pneumococcal invasive disease is triggered by initial asymptomatic colonization of the human upper respiratory tract. The pneumococcal serine-...

247 research outcomes, page 1 of 25
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