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  • Publication . Article . 2016 . Embargo End Date: 31 Oct 2016
    English
    Authors: 
    Forment, Josep; Herzog, Mareike; Coates, Julia; Konopka, T; Gapp, BV; Nijman, SM; Adams, DJ; Keane, TM; Jackson, Stephen;
    Publisher: Apollo - University of Cambridge Repository
    Country: United Kingdom

    Research in the S.P.J. laboratory is funded by Cancer Research UK (CRUK; programme grant C6/A11224), the European Research Council and the European Community Seventh Framework Programme (grant agreement no. HEALTH-F2-2010-259893; DDResponse). Core funding is provided by...

  • Open Access
    Authors: 
    Owen R. Davies; Josep V. Forment; Meidai Sun; Rimma Belotserkovskaya; Julia Coates; Yaron Galanty; Mukerrem Demir; Christopher R Morton; Neil J. Rzechorzek; Stephen P. Jackson; +1 more
    Publisher: Springer Science and Business Media LLC
    Country: United Kingdom
    Project: WT | Core support for the Well... (092096), EC | DDRESPONSE (259893)

    Mammalian CtIP protein has major roles in DNA double-strand break (DSB) repair. Although it is well established that CtIP promotes DNA-end resection in preparation for homology-dependent DSB repair, the molecular basis for this function has remained unknown. Here we sho...

  • Open Access English
    Authors: 
    Josep V. Forment; Stephen P. Jackson;
    Country: United Kingdom

    Protein accumulation on chromatin has traditionally been studied using immunofluorescence microscopy or biochemical cellular fractionation followed by western immunoblot analysis. As a way to improve the reproducibility of this kind of analysis, to make it easier to qua...

  • Open Access English
    Authors: 
    Gabriel Balmus; Natasha A. Karp; Bee Ling Ng; Stephen P. Jackson; David J. Adams; Rebecca E. McIntyre;
    Country: United Kingdom

    We describe a sensitive, robust, high-throughput method for quantifying the formation of micronuclei, markers of genome instability, in mouse erythrocytes. Micronuclei are whole chromosomes or chromosome segments that have been separated from the nucleus. Other methods ...

  • English
    Authors: 
    Ochi, Takashi; Blackford, Andrew; Coates, Julia; Jhujh, Satpal; Mehmood, Shahid; Tamura, Naoka; Travers, Jon; Wu, Qian; Draviam Sastry, Viji; Robinson, Carol V; +2 more
    Publisher: American Association for the Advancement of Science (AAAS)
    Country: United Kingdom

    XRCC4 and XLF are two structurally related proteins that function in DNA double-strand break (DSB) repair. Here, we identify human PAXX (PAralog of XRCC4 and XLF, also called C9orf142) as a new XRCC4 superfamily member and show that its crystal structure resembles that ...

  • Open Access
    Authors: 
    Jessica S. Brown; Stephen P. Jackson;
    Publisher: The Royal Society
    Country: United Kingdom
    Project: WT | Core support for the Well... (092096), EC | DDRESPONSE (259893), WT | Clinical PhD Programme at... (094794)

    Failure of accurate DNA damage sensing and repair mechanisms manifests as a variety of human diseases, including neurodegenerative disorders, immunodeficiency, infertility and cancer. The accuracy and efficiency of DNA damage detection and repair, collectively termed th...

  • Open Access English
    Authors: 
    Puddu, Fabio; Salguero, I; Herzog, Mareike; Geisler, NJ; Costanzo, V; Jackson, Stephen;
    Publisher: John Wiley and Sons Inc.
    Country: United Kingdom
    Project: WT | Mutational signatures of ... (101126), WT | Core support for the Well... (092096), WT | Wellcome Trust Sanger Ins... (098051)

    Camptothecin-induced locking of topoisomerase 1 on DNA generates a physical barrier to replication fork progression and creates topological stress. By allowing replisome rotation, absence of the Tof1/Csm3 complex promotes the conversion of impending topological stress t...

  • Open Access English
    Authors: 
    Chanut, Pauline; Britton, Sébastien; Coates, Julia; Jackson, Stephen P.; Calsou, Patrick;
    Publisher: Nature Portfolio
    Country: United Kingdom
    Project: WT | Core support for the Well... (092096)

    Repair of single-ended DNA double-strand breaks (seDSBs) by homologous recombination (HR) requires the generation of a 3' single-strand DNA overhang by exonuclease activities in a process called DNA resection. However, it is anticipated that the highly abundant DNA end-...

  • Publication . Article . Other literature type . 2015
    Open Access English
    Authors: 
    Christine K. Schmidt; Yaron Galanty; Matylda Sczaniecka-Clift; Julia Coates; Satpal Jhujh; Mukerrem Demir; Matthew Cornwell; Petra Beli; Stephen P. Jackson;
    Publisher: Springer Science and Business Media LLC
    Country: United Kingdom

    Ubiquitylation is crucial for proper cellular responses to DNA double-strand breaks (DSBs). If unrepaired, these highly cytotoxic lesions cause genome instability, tumorigenesis, neurodegeneration or premature ageing. Here, we conduct a comprehensive, multilayered scree...

  • Open Access English
    Authors: 
    Chang, HHY; Watanabe, G; Gerodimos, CA; Ochi, T; Blundell, Tom; Jackson, Stephen; Lieber, MR;
    Publisher: American Society for Biochemistry and Molecular Biology
    Country: United Kingdom
    Project: NIH | MECHANISM AND REGULATION ... (5R01CA100504-03), WT | Core support for the Well... (092096), WT | Structural Biology of DNA... (093167), NIH | Site-Specific Recombinati... (5R35GM118009-02)

    The nonhomologous DNA end-joining (NHEJ) pathway is a key mechanism for repairing dsDNA breaks that occur often in eukaryotic cells. In the simplest model, these breaks are first recognized by Ku, which then interacts with other NHEJ proteins to improve their affinity a...