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Heteroplasmy Benchmark Dataset - mitochondrial DNA mixture model - MiSeq - U5-H1-M1-M2-M3-M4-M5 - BAM

Name: Heteroplasmy Benchmark Dataset - mitochondrial DNA mixture model - MiSeq - U5-H1-M1-M2-M3-M4-M5 - BAM
Keywords: polymerase, benchmark, Illumina, mtdna, mixture-model, ngs, miseq, MiSeq, illumina, heteroplasmy

Research datakeyboard_double_arrow_right Dataset 01 Jan 2020Embargo end date: 01 Jan 2021Publisher:Zenodo

Authors: Federica Fazzini; Liane Fendt; Lukas Forer; Sebastian Schönherr; Jamie Lee Losso; Gertraud Streiter; Bernd Schöpf; +3 Authors

doi: 10.5281/zenodo.4001423 , 10.5281/zenodo.3991748 , 10.5281/zenodo.3991749 , 10.5281/zenodo.4001424 , 10.5281/zenodo.4395665

Heteroplasmy Benchmark Dataset - mitochondrial DNA mixture model - MiSeq - U5-H1-M1-M2-M3-M4-M5 - BAM

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Abstract

mtDNA mixture model of 2 mtDNA sequences belonging to haplogroups U5 and H1. Run on Illumina MiSeq with 3 different polymerases (Clontech, Herculase, NEB Taq), and different DNA extraction protocols - BAM FILES M1 = Mixture 1:2 i.e. 50% M2 = Mixture 1:10 i.e. 10% M3 = Mixture 1:50 i.e. 2% M4 = Mixture 1:100 i.e. 1% M5 = Mixture 1:200 i.e. 0.5%

Related Organizations

Innsbruck Medical University
Austria

Keywords

polymerase, benchmark, Illumina, mtdna, mixture-model, ngs, miseq, MiSeq, illumina, heteroplasmy, next-generation sequencing

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