research data . Dataset . 2019

Analysis of dephosphorylated HTT samples for cryoEM analysis and further sample preparation 2019/04/15

Harding, Rachel; Deme, Justin; Lea, Susan; Arrowsmith, Cheryl; Edwards, Aled;
Open Access
  • Published: 15 Apr 2019
  • Publisher: Zenodo
Abstract
<p><strong>Project:&nbsp;</strong>High resolution structural analysis of purified HTT samples</p> <p><strong>Experiment:&nbsp;</strong>Analysis of dephosphorylated HTT samples for cryoEM analysis and further sample preparation</p> <p><strong>Date completed:&shy;&nbsp;</strong>2019/04/15</p> <p><strong>Rationale:&nbsp;</strong>We now have a good understanding of the global structure of HTT when in complex with HAP40 and our MALS and SAXS data suggest that apo HTT is likely self-associating and heterogenous in nature, despite high levels of purity. Dephosphorylating HTT from Sf9 cell production could alter its global structure sufficiently to allow high resolution...
Subjects
free text keywords: huntingtin, HTT, Huntington's disease, HD, cryoEM, Phosphorylation, Biophysics, Biochemistry, Genetics, Molecular Biology, Biotechnology, Science Policy, 69999 Biological Sciences not elsewhere classified, 39999 Chemical Sciences not elsewhere classified, 80699 Information Systems not elsewhere classified, Biological Sciences not elsewhere classified, Chemical Sciences not elsewhere classified, Information Systems not elsewhere classified
Communities
Science and Innovation Policy Studies
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Dataset . 2019
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Dataset . 2019
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Dataset . 2019
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Dataset . 2019
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