research data . Dataset . 2019

Analysis of dephosphorylated HTT samples by cryoEM analysis 2019/05/01

Harding, Rachel; Deme, Justin; Lea, Susan; Arrowsmith, Cheryl; Edwards, Aled;
Open Access
  • Published: 01 May 2019
  • Publisher: Zenodo
Abstract
<p><strong>Project:&nbsp;</strong>High resolution structural analysis of purified HTT samples</p> <p><strong>Experiment:&nbsp;</strong>Analysis of dephosphorylated HTT samples by cryoEM analysis&nbsp;</p> <p><strong>Date completed:&shy;&nbsp;</strong>2019/05/01</p> <p><strong>Rationale:&nbsp;</strong>We now have a good understanding of the global structure of HTT when in complex with HAP40 and our MALS and SAXS data suggest that apo HTT is likely self-associating and heterogenous in nature, despite high levels of purity. Dephosphorylating HTT from Sf9 cell production could alter its global structure sufficiently to allow high resolution structure determination b...
Subjects
free text keywords: Huntingtin, HTT, HD, Huntington's disease, CryoEM, Phosphorylation
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Zenodo
Dataset . 2019
Provider: Datacite
Zenodo
Dataset . 2019
Provider: Datacite
Zenodo
Dataset . 2019
Provider: Zenodo
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research data . Dataset . 2019

Analysis of dephosphorylated HTT samples by cryoEM analysis 2019/05/01

Harding, Rachel; Deme, Justin; Lea, Susan; Arrowsmith, Cheryl; Edwards, Aled;