research data . Dataset . 2019

Large-scale stringent purification of Q23 HTT and HTT-HAP40 using heparin affinity chromatography 2019/05/22

Harding, Rachel; Alvarez, Claudia; McAuley, Jacob; Hutchinson, Ashley; Seitova, Alma; Arrowsmith, Cheryl; Edwards, Aled;
Open Access
  • Published: 22 May 2019
  • Publisher: Zenodo
Abstract
<p><strong>Project:&nbsp;</strong>Biophysical investigation of purified HTT protein samples</p> <p><strong>Experiment:&nbsp;</strong>Large-scale stringent purification of Q23 HTT and HTT-HAP40 using heparin affinity chromatography</p> <p><strong>Date completed:&shy;&nbsp;</strong>2019/05/22</p> <p><strong>Rationale:&nbsp;</strong>Previous attempts to generate a much purer and homogenous HTT-HAP40 sample showed that the complex can bind heparin resin. In small-scale experiment, I also incorporated helpful suggestions from scientists at the CHDI Palm Springs meeting full-length HTT research breakout group i.e. ATP wash to remove HSP proteins. The experiment includ...
Subjects
Medical Subject Headings: congenital, hereditary, and neonatal diseases and abnormalitiesmental disorders
free text keywords: Huntingtin, HTT, HAP40, Huntington's disease, HD
Download fromView all 3 versions
Zenodo
Dataset . 2019
Provider: Zenodo
Zenodo
Dataset . 2019
Provider: Datacite
Zenodo
Dataset . 2019
Provider: Datacite
Powered by OpenAIRE Open Research Graph
Any information missing or wrong?Report an Issue
research data . Dataset . 2019

Large-scale stringent purification of Q23 HTT and HTT-HAP40 using heparin affinity chromatography 2019/05/22

Harding, Rachel; Alvarez, Claudia; McAuley, Jacob; Hutchinson, Ashley; Seitova, Alma; Arrowsmith, Cheryl; Edwards, Aled;