Large scale expression and purification of full-length huntingtin Q23 with HAP40 from baculoviral expression system production in sf9 insect cells – 2018/05/28

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Harding, Rachel ; Seitova, Alma ; Hutchinson, Ashley ; Arrowsmith, Cheryl ; Edwards, Aled
  • Publisher: Zenodo
  • Related identifiers: doi: 10.5281/zenodo.1305940
  • Subject: Huntingtin | HTT | HAP40 | Protein-protein interaction | SAXS

<p><strong>Project</strong>&nbsp;- Huntingtin structure-function open lab notebook.&nbsp;</p> <p><strong>Experiment and rationale</strong>&nbsp;-&nbsp;Purified huntingtin samples with stabilising binding partners (e.g. HAP40) are required for use in structural and functional studies, in particular SAXS experiments.&nbsp;</p>
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