research data . Dataset . 2019

Cloning and test expression of HTT fragment clones - 2019/02/28

Harding, Rachel; Hutchinson, Ashley; Seitova, Alma; Arrowsmith, Cheryl; Lee, Matt;
Open Access
  • Published: 28 Feb 2019
  • Publisher: Zenodo
Abstract
<strong>Project: </strong>High resolution structural analysis of purified HTT proteins <strong>Experiment: </strong>Cloning and test expression of HTT fragment clones <strong>Date completed:­ </strong>2019/02/28 <strong>Rationale: </strong>I aim to complement our previous work on purifying stable regions of huntingtin by cloning further regions of huntingtin corresponding to discrete HEAT repeats for eukaryotic expression. Constructs will initially be screened by BVES in insect sf9 cells. High quality constructs will undergo extensive crystallization experiments and structure determination by X-ray crystallography using well established high-throughput and syste...
Subjects
Medical Subject Headings: mental disorderscongenital, hereditary, and neonatal diseases and abnormalities
free text keywords: Huntingtin, Protein expression, Protein purification, X-ray crystallography
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Zenodo
Dataset . 2019
Provider: Zenodo
Zenodo
Dataset . 2019
Provider: Datacite
Zenodo
Dataset . 2019
Provider: Datacite
Zenodo
Dataset . 2019
Provider: Datacite
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