research data . Dataset . 2019

Preparation of dephosphorylated HTT samples for cryoEM analysis 2019/03/20

Harding, Rachel; Duan, Shili; Arrowsmith, Cheryl;
Open Access
  • Published: 20 Mar 2019
  • Publisher: Zenodo
Abstract
<p><strong>Project:&nbsp;</strong>High resolution structural analysis of purified HTT samples</p> <p><strong>Experiment:&nbsp;</strong>Preparation of dephosphorylated HTT samples for cryoEM analysis</p> <p><strong>Date completed:&shy;&nbsp;</strong>2019/03/20</p> <p><strong>Rationale:&nbsp;</strong>We now have a good understanding of the global structure of HTT when in complex with HAP40 and our MALS and SAXS data suggest that apo HTT is likely self-associating and heterogenous in nature, despite high levels of purity. Dephosphorylating HTT from Sf9 cell production could alter its global structure sufficiently to allow high resolution structure determination by ...
Subjects
free text keywords: Huntingtin, Huntington's disease, HTT, GRAFIX, CryoEM, Biophysics, Biochemistry, Genetics, Pharmacology, Biotechnology, 39999 Chemical Sciences not elsewhere classified, 80699 Information Systems not elsewhere classified
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Dataset . 2019
Provider: Datacite
Zenodo
Dataset . 2019
Provider: Zenodo
Zenodo
Dataset . 2019
Provider: Datacite
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Dataset . 2019
Provider: figshare
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