research data . Dataset . 2019

Preparation of dephosphorylated HTT samples for cryoEM analysis 2019/03/20

Harding, Rachel; Duan, Shili; Arrowsmith, Cheryl;
Open Access
  • Published: 20 Mar 2019
  • Publisher: Zenodo
Abstract
<p><strong>Project:&nbsp;</strong>High resolution structural analysis of purified HTT samples</p> <p><strong>Experiment:&nbsp;</strong>Preparation of dephosphorylated HTT samples for cryoEM analysis</p> <p><strong>Date completed:&shy;&nbsp;</strong>2019/03/20</p> <p><strong>Rationale:&nbsp;</strong>We now have a good understanding of the global structure of HTT when in complex with HAP40 and our MALS and SAXS data suggest that apo HTT is likely self-associating and heterogenous in nature, despite high levels of purity. Dephosphorylating HTT from Sf9 cell production could alter its global structure sufficiently to allow high resolution structure determination by ...
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Persistent Identifiers

doi: 10.5281/zenodo.2601986 , 10.5281/zenodo.2601987

Subjects
free text keywords: Huntingtin, Huntington's disease, HTT, GRAFIX, CryoEM, Biophysics, Biochemistry, Genetics, Pharmacology, Biotechnology, 39999 Chemical Sciences not elsewhere classified, 80699 Information Systems not elsewhere classified
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Dataset . 2019
Provider: Datacite
Zenodo
Dataset . 2019
Provider: Zenodo
Zenodo
Dataset . 2019
Provider: Datacite
figshare
Dataset . 2019
Provider: figshare
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