research data . Dataset . 2019

Optimisation of HTT-HAP40 purification using heparin affinity chromatography (2019/01/14)

Harding, Rachel; Hutchinson, Ashley; Seitova, Alma; Arrowsmith, Cheryl; Edwards, Aled;
Open Access
  • Published: 14 Jan 2019
  • Publisher: Zenodo
Abstract
<p><strong>Project</strong>&nbsp;- Huntingtin structure-function open lab notebook. &nbsp;</p> <p><strong>Experiment</strong>&nbsp;- Optimisation of HTT-HAP40 purification using heparin affinity chromatography.</p> <p><strong>Aims</strong> -&nbsp;The current protocol for HTT and HTT-HAP40 purification I am using requires a long incubation of clarified cell lysate with FLAG resin.&nbsp;To potentially improve yields and sample quality, it would perhaps be beneficial to have a quick heparin resin purification step prior to FLAG binding which may also remove contaminating nucleic acid material. To test this hypothesis, small-scale purification of Q23 HTT-HAP40 sampl...
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doi: 10.5281/zenodo.2553669 , 10.5281/zenodo.2553668
Subjects
Medical Subject Headings: congenital, hereditary, and neonatal diseases and abnormalitiesmental disorders
free text keywords: Huntingtin, HAP40, Protein purification, Huntington's disease, Biophysics, Biochemistry, Medicine, Cell Biology, Genetics, Neuroscience, Pharmacology, Biotechnology, 39999 Chemical Sciences not elsewhere classified, 80699 Information Systems not elsewhere classified
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Dataset . 2019
Provider: Zenodo
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Dataset . 2019
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Dataset . 2019
Provider: Datacite
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Dataset . 2019
Provider: figshare
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