research data . Dataset . 2016

Connecting Quorum Sensing, c-di-GMP, Pel Polysaccharide, and Biofilm Formation in Pseudomonas aeruginosa through Tyrosine Phosphatase TpbA (PA3885)

Ueda, Akihiro; Wood, Thomas K.;
  • Published: 01 Jan 2016
  • Publisher: Figshare
Abstract
<div><p>With the opportunistic pathogen <em>Pseudomonas aeruginosa</em>, quorum sensing based on homoserine lactones was found to influence biofilm formation. Here we discern a mechanism by which quorum sensing controls biofilm formation by screening 5850 transposon mutants of <em>P. aeruginosa</em> PA14 for altered biofilm formation. This screen identified the PA3885 mutant, which had 147-fold more biofilm than the wild-type strain. Loss of PA3885 decreased swimming, abolished swarming, and increased attachment, although this did not affect production of rhamnolipids. The PA3885 mutant also had a wrinkly colony phenotype, formed pronounced pellicles, had substa...
Subjects
Medical Subject Headings: biochemical phenomena, metabolism, and nutrition
free text keywords: Microbiology, Cell Biology, Genetics, Molecular Biology, Cancer, connecting, quorum, pel, biofilm, tyrosine, phosphatase, tpba
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Dataset . 2016
Provider: figshare
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