A Next-Generation Sequencing Method for Genotyping-by-Sequencing of Highly Heterozygous Autotetraploid Potato

Dataset UNKNOWN
G. A. M. L. Uitdewilligen, Jan; A. Wolters, Anne-Marie; B. D’hoop, Bjorn; J. A. Borm, Theo; G. F. Visser, Richard; J. van Eck, Herman;
(2016)
  • Publisher: Figshare
  • Identifiers: doi: 10.1371/journal.pone.0062355
  • Subject: Molecular Biology | alternative allele copy number states | Next-Generation Sequencing Method | autotetraploid potato cultivars | DM | in-solution hybridisation method | Ecology | potato reference genome | Genetics | Evolutionary Biology | MAF | variant | genomic DNA sequence variation | allele copy number | 83 tetraploid cultivars | sequence data | allele copy number estimates | 807 target genes | Microbiology | 0.14. Potato germplasm | cultivar-specific DNA sequencing libraries | Heterozygous Autotetraploid Potato Assessment | KASP genotyping assay | depth | SNP | Illumina HiSeq 2000. | GBS
    • FOR: 59999 Environmental Sciences not elsewhere classified | 69999 Biological Sciences not elsewhere classified | 60506 Virology
    mesheuropmc: food and beverages

<div><p>Assessment of genomic DNA sequence variation and genotype calling in autotetraploids implies the ability to distinguish among five possible alternative allele copy number states. This study demonstrates the accuracy of genotyping-by-sequencing (GBS) of a large c... View more
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