research data . Dataset . 2016

Accumulation of DNA Damage in Hematopoietic Stem and Progenitor Cells during Human Aging

E. Rübe, Claudia; Fricke, Andreas; A. Widmann, Thomas; Fürst, Tobias; Madry, Henning; Pfreundschuh, Michael; Rübe, Christian;
  • Published: 01 Jan 2016
  • Publisher: Figshare
Abstract
<div><h3>Background</h3><p>Accumulation of DNA damage leading to adult stem cell exhaustion has been proposed to be a principal mechanism of aging. Here we tested this hypothesis in healthy individuals of different ages by examining unrepaired DNA double-strand breaks (DSBs) in hematopoietic stem/progenitor cells matured in their physiological microenvironment.</p> <h3>Methodology/Principal Findings</h3><p>To asses DNA damage accumulation and repair capacities, γH2AX-foci were examined before and after exposure to ionizing irradiation. Analyzing CD34+ and CD34− stem/progenitor cells we observed an increase of endogenous γH2AX-foci levels with advancing donor age...
Subjects
free text keywords: Molecular Biology, Hematology, accumulation, dna, hematopoietic, progenitor, cells, aging
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Dataset . 2016
Provider: figshare
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