research data . Dataset . 2016

Biased Diversity Metrics Revealed by Bacterial 16S Pyrotags Derived from Different Primer Sets

Cai, Lin; Ye, Lin; Hin Yan Tong, Amy; Lok, Si; Zhang, Tong;
  • Published: 01 Jan 2016
  • Publisher: Figshare
Abstract
<div><p>In recent years, PCR-based pyrosequencing of 16S rRNA genes has continuously increased our understanding of complex microbial communities in various environments of the Earth. However, there is always concern on the potential biases of diversity determination using different 16S rRNA gene primer sets and covered regions. Here, we first report how bacterial 16S rRNA gene pyrotags derived from a series of different primer sets resulted in the biased diversity metrics. In total, 14 types of pyrotags were obtained from two-end pyrosequencing of 7 amplicon pools generated by 7 primer sets paired by 1 of 4 forward primers (V1F, V3F, V5F, and V7F) and 1 of 4 re...
Subjects
free text keywords: Microbiology, Biotechnology, Evolutionary Biology, biased, metrics, revealed, bacterial, 16s, pyrotags, derived, primer, sets
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Dataset . 2016
Provider: figshare
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