Functionalized Micromachines for Selective and Rapid Isolation of Nucleic Acid Targets from Complex Samples

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Kagan, Daniel ; Campuzano, Susana ; Balasubramanian, Shankar ; Kuralay, Filiz ; Flechsig, Gerd-Uwe ; Wang, Joseph
  • Publisher: Figshare
  • Related identifiers: doi: 10.1021/nl2005687.s008
  • Subject: Biophysics | Medicine | Microbiology | Molecular Biology | Evolutionary Biology | Ecology | Immunology | Inorganic Chemistry | Space Science | crude E | sample | acid targets | Complex SamplesThe transport properties | fluid convection | Rapid Isolation | DNA | hybridization efficiency | Functionalized Micromachines | coli lysate | microrocket | acid isolation
    • FOR: 69999 Biological Sciences not elsewhere classified | 39999 Chemical Sciences not elsewhere classified

The transport properties of single-strand DNA probe-modified self-propelling micromachines are exploited for “on-the-fly” hybridization and selective single-step isolation of target nucleic acids from “raw” microliter biological samples (serum, urine, crude <i>E. coli</i> lysate, saliva). The rapid movement of the guided modified microrockets induces fluid convection, which enhances the hybridization efficiency, thus enabling the rapid and selective isolation of nucleic acid targets from untreated samples. The integration of these autonomous microrockets into a lab-on-chip device that provides both nucleic acid isolation and downstream analysis could thus be attractive for diverse applications.
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