research data . Dataset . 2016

Dengue-3 Virus Entry into Vero Cells: Role of Clathrin-Mediated Endocytosis in the Outcome of Infection

E. Piccini, Luana; Castilla, Viviana; B. Damonte, Elsa;
  • Published: 01 Jan 2016
  • Publisher: Figshare
Abstract
<div><p>The endocytic uptake and intracellular trafficking for penetration of DENV-3 strain H-87 into Vero cells was analyzed by using several biochemical inhibitors and dominant negative mutants of cellular proteins. The results presented show that the infective entry of DENV-3 into Vero cells occurs through a non-classical endocytosis pathway dependent on low pH and dynamin, but non-mediated by clathrin. After uptake, DENV-3 transits through early endosomes to reach Rab 7-regulated late endosomes, and according with the half-time for ammonium chloride resistance viral nucleocapsid is released into the cytosol approximately at 12 min post-infection. Furthermore...
Subjects
Medical Subject Headings: viruses
free text keywords: Uncategorised, U 937 cells, denv, ammonium chloride resistance, host cell, infective, Vero cells, pathway, entry
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Dataset . 2016
Provider: figshare
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