research data . Dataset . 2016

Mitochondrial Respiration in Insulin-Producing β-Cells: General Characteristics and Adaptive Effects of Hypoxia

K. Hals, Ingrid; Gustafson Bruerberg, Simon; Ma, Zuheng; Scholz, Hanne; Björklund, Anneli; Grill, Valdemar;
  • Published: 01 Jan 2016
  • Publisher: Figshare
Abstract
<div><p>Objective</p><p>To provide novel insights on mitochondrial respiration in β-cells and the adaptive effects of hypoxia.</p><p>Methods and Design</p><p>Insulin-producing INS-1 832/13 cells were exposed to 18 hours of hypoxia followed by 20–22 hours re-oxygenation. Mitochondrial respiration was measured by high-resolution respirometry in both intact and permeabilized cells, in the latter after establishing three functional substrate-uncoupler-inhibitor titration (SUIT) protocols. Concomitant measurements included proteins of mitochondrial complexes (Western blotting), ATP and insulin secretion.</p><p>Results</p><p>Intact cells exhibited a high degree of int...
Subjects
free text keywords: Uncategorised, hypoxia, substrate, atp, SUIT protocols, capacity, respiration, ins, insulin secretion.ResultsIntact cells, mitochondrial complexes, ii
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Dataset . 2016
Provider: figshare
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