research data . Dataset . 2018

Table_3_Transformation of Penicillium rubens 212 and Expression of GFP and DsRED Coding Genes for Visualization of Plant-Biocontrol Agent Interaction.PDF

Villarino, Maria; A. Espeso, Eduardo; Melgarejo, Paloma; Larena, Inmaculada;
  • Published: 01 Jan 2018
  • Publisher: Figshare
Abstract
<p>Strain 212 of Penicillium rubens (PO212) is an effective fungal biological control agent against a broad spectrum of diseases of horticultural plants. A pyrimidine auxotrophic isolate of PO212, PO212_18.2, carrying an inactive pyrG gene, has been used as host for transformation by positive selection of vectors containing the gene complementing the pyrG1 mutation. Both integrative and autonomously replicating plasmids transformed PO212_18.2 with high efficiency. Novel PO212-derived strains expressed green (sGFP) and red (Ds-Red Express) fluorescent reporter proteins, driven by the A. nidulans gpdA promoter. Fluorescence microscopy revealed constitutive express...
Subjects
Medical Subject Headings: food and beverages
free text keywords: Microbiology, 60503 Microbial Genetics, 60504 Microbial Ecology, 60505 Mycology, DsRed, GFP, fluorescent reporter, biocontrol, root colonization, fungal transformation, autonomous replication
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Dataset . 2018
Provider: figshare
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