research data . Dataset . 2016

Reversible Dissolution of Microdomains in Detergent-Resistant Membranes at Physiological Temperature

Cremona, Andrea; Orsini, Francesco; A. Corsetto, Paola; Hoogenboom, Bart W.; M. Rizzo, Angela;
  • Published: 01 Jan 2016
  • Publisher: Figshare
Abstract
<div><p>The formation of lipid microdomains (“rafts”) is presumed to play an important role in various cellular functions, but their nature remains controversial. Here we report on microdomain formation in isolated, detergent-resistant membranes from MDA-MB-231 human breast cancer cells, studied by atomic force microscopy (AFM). Whereas microdomains were readily observed at room temperature, they shrunk in size and mostly disappeared at higher temperatures. This shrinking in microdomain size was accompanied by a gradual reduction of the height difference between the microdomains and the surrounding membrane, consistent with the behaviour expected for lipids that...
Subjects
Medical Subject Headings: endocrine system
free text keywords: Biological Sciences, microdomain size, height difference, microdomains reversibly, force microscopy, afm, Immunolabeling experiments, Physiological Temperature, membrane order, room temperature, microdomain formation, Reversible Dissolution, breast cancer cells, lipid rafts
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Dataset . 2016
Provider: figshare
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