research data . Dataset . 2016

Brg1 Is Required for Cdx2-Mediated Repression of Oct4 Expression in Mouse Blastocysts

Wang, Kai; Sengupta, Satyaki; Magnani, Luca; A. Wilson, Catherine; Henry, R. William; G. Knott, Jason;
  • Published: 01 Jan 2016
  • Publisher: Figshare
Abstract
<div><p>During blastocyst formation the segregation of the inner cell mass (ICM) and trophectoderm is governed by the mutually antagonistic effects of the transcription factors Oct4 and Cdx2. Evidence indicates that suppression of Oct4 expression in the trophectoderm is mediated by Cdx2. Nonetheless, the underlying epigenetic modifiers required for Cdx2-dependent repression of Oct4 are largely unknown. Here we show that the chromatin remodeling protein Brg1 is required for Cdx2-mediated repression of Oct4 expression in mouse blastocysts. By employing a combination of RNA interference (RNAi) and gene expression analysis we found that both Brg1 Knockdown (KD) and ...
Subjects
Medical Subject Headings: embryonic structuresreproductive and urinary physiologydigestive system diseasesurogenital system
free text keywords: Molecular Biology, Developmental Biology, brg1, cdx2-mediated, repression, oct4, blastocysts
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Dataset . 2016
Provider: figshare
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