research data . Dataset . 2016

A Novel Method for Detection of Phosphorylation in Single Cells by Surface Enhanced Raman Scattering (SERS) using Composite Organic-Inorganic Nanoparticles (COINs)

M. Shachaf, Catherine; V. Elchuri, Sailaja; Koh, Ai Leen; Zhu, Jing; Nguyen, Lienchi N.; J. Mitchell, Dennis; Zhang, Jingwu; B. Swartz, Kenneth; Sun, Lei; Chan, Selena; ...
  • Published: 01 Jan 2016
  • Publisher: Figshare
Abstract
<div><h3>Background</h3><p>Detection of single cell epitopes has been a mainstay of immunophenotyping for over three decades, primarily using fluorescence techniques for quantitation. Fluorescence has broad overlapping spectra, limiting multiplexing abilities.</p><h3>Methodology/Principal Findings</h3><p>To expand upon current detection systems, we developed a novel method for multi-color immuno-detection in single cells using “Composite Organic-Inorganic Nanoparticles” (COINs) Raman nanoparticles. COINs are Surface-Enhanced Raman Scattering (SERS) nanoparticles, with unique Raman spectra. To measure Raman spectra in single cells, we constructed an automated, co...
Subjects
free text keywords: Medicine, Cell Biology, Cancer, detection, phosphorylation, cells, enhanced, raman, scattering, composite, organic-inorganic, nanoparticles
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Dataset . 2016
Provider: figshare
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