<script type="text/javascript">
<!--
document.write('<div id="oa_widget"></div>');
document.write('<script type="text/javascript" src="https://www.openaire.eu/index.php?option=com_openaire&view=widget&format=raw&projectId=undefined&type=result"></script>');
-->
</script>

COPY SCRIPT

For further information contact us at helpdesk@openaire.eu

Coexpression and purification of HTT domains with HAP40 - 2018/12/03

Research datakeyboard_double_arrow_right Dataset 03 Dec 2018Publisher:Zenodo

Authors: Harding, Rachel; Hutchinson, Ashley; Seitova, Alma; Arrowsmith, Cheryl;

doi: 10.5281/zenodo.2600233 , 10.5281/zenodo.2600232

Coexpression and purification of HTT domains with HAP40 - 2018/12/03

- Summary
- Subjects
- Related research
  (13)
- Metrics

Abstract

Dr. Harding is the recipient of the Huntington's Disease Society of America Berman Topper Career Development Fellowship which funds and supports this research, in addition to generous funding from the CHDI Foundation and the Huntington Society of Canada. The SGC is a registered charity (number 1097737) that receives funds from AbbVie, Bayer Pharma AG, Boehringer Ingelheim, Canada Foundation for Innovation, Eshelman Institute for Innovation, Genome Canada through Ontario Genomics Institute [OGI-055], Innovative Medicines Initiative (EU/EFPIA) [ULTRA-DD grant no. 115766], Janssen, Merck KGaA, Darmstadt, Germany, MSD, Novartis Pharma AG, Ontario Ministry of Research, Innovation and Science (MRIS), Pfizer, São Paulo Research Foundation-FAPESP, Takeda, and Wellcome.

Project: High resolution structural analysis of purified HTT proteins Experiment: Coexpression and purification of HTT domains with HAP40 Date completed: 2018/12/03 Rationale: HTT domain fragments are challenging to express and purify. However, the full-length protein sample was shown to have superior biophysical characteristics when coexpressed and purified with HAP40. As HAP40 has an extensive interaction surface with HTT, it could be possible to copurify domain fragments of HTT with HAP40, stabilizing the individual HEAT domains.

Related Organizations

University of Toronto
Canada

Keywords

Protein purification, HAP40, Huntingtin, HTT

13 Research products, page 1 of 2

Analysis of dephosphorylated HTT samples by cryoEM analysis 2019/05/01
Research data2019IsAmongTopNSimilarDocuments
linkLink to
shareshareShare
citeCite
addClaim
Please grant OpenAIRE to access and update your ORCID works.
This Research product is the result of merged Research products in OpenAIRE.

You have already added works in your ORCID record related to the merged Research product.
This Research product is the result of merged Research products in OpenAIRE.

You have already added works in your ORCID record related to the merged Research product.
Select content type to embed
All Research products
arrow_drop_down
<script type="text/javascript">  </script>
COPY SCRIPT
For further information contact us at helpdesk@openaire.eu
more_vert
linkLink to
shareshareShare
citeCite
addClaim
Please grant OpenAIRE to access and update your ORCID works.
This Research product is the result of merged Research products in OpenAIRE.

You have already added works in your ORCID record related to the merged Research product.
This Research product is the result of merged Research products in OpenAIRE.

You have already added works in your ORCID record related to the merged Research product.
Select content type to embed
All Research products
arrow_drop_down
<script type="text/javascript">  </script>
COPY SCRIPT
For further information contact us at helpdesk@openaire.eu
Large-scale purification of HTT domain constructs 2019/04/01
Research data2019IsAmongTopNSimilarDocuments
linkLink to
shareshareShare
citeCite
addClaim
Please grant OpenAIRE to access and update your ORCID works.
This Research product is the result of merged Research products in OpenAIRE.

You have already added works in your ORCID record related to the merged Research product.
This Research product is the result of merged Research products in OpenAIRE.

You have already added works in your ORCID record related to the merged Research product.
Select content type to embed
All Research products
arrow_drop_down
<script type="text/javascript">  </script>
COPY SCRIPT
For further information contact us at helpdesk@openaire.eu
more_vert
linkLink to
shareshareShare
citeCite
addClaim
Please grant OpenAIRE to access and update your ORCID works.
This Research product is the result of merged Research products in OpenAIRE.

You have already added works in your ORCID record related to the merged Research product.
This Research product is the result of merged Research products in OpenAIRE.

You have already added works in your ORCID record related to the merged Research product.
Select content type to embed
All Research products
arrow_drop_down
<script type="text/javascript">  </script>
COPY SCRIPT
For further information contact us at helpdesk@openaire.eu
Large-scale purification of Q23 HTT from Sf9 2019/09/30
Research data2019IsAmongTopNSimilarDocuments
linkLink to
shareshareShare
citeCite
addClaim
Please grant OpenAIRE to access and update your ORCID works.
This Research product is the result of merged Research products in OpenAIRE.

You have already added works in your ORCID record related to the merged Research product.
This Research product is the result of merged Research products in OpenAIRE.

You have already added works in your ORCID record related to the merged Research product.
Select content type to embed
All Research products
arrow_drop_down
<script type="text/javascript">  </script>
COPY SCRIPT
For further information contact us at helpdesk@openaire.eu
more_vert
linkLink to
shareshareShare
citeCite
addClaim
Please grant OpenAIRE to access and update your ORCID works.
This Research product is the result of merged Research products in OpenAIRE.

You have already added works in your ORCID record related to the merged Research product.
This Research product is the result of merged Research products in OpenAIRE.

You have already added works in your ORCID record related to the merged Research product.
Select content type to embed
All Research products
arrow_drop_down
<script type="text/javascript">  </script>
COPY SCRIPT
For further information contact us at helpdesk@openaire.eu
Analysis of dephosphorylated HTT samples for cryoEM analysis and further sample preparation 2019/04/15
Research data2019IsAmongTopNSimilarDocuments
linkLink to
shareshareShare
citeCite
addClaim
Please grant OpenAIRE to access and update your ORCID works.
This Research product is the result of merged Research products in OpenAIRE.

You have already added works in your ORCID record related to the merged Research product.
This Research product is the result of merged Research products in OpenAIRE.

You have already added works in your ORCID record related to the merged Research product.
Select content type to embed
All Research products
arrow_drop_down
<script type="text/javascript">  </script>
COPY SCRIPT
For further information contact us at helpdesk@openaire.eu
more_vert
linkLink to
shareshareShare
citeCite
addClaim
Please grant OpenAIRE to access and update your ORCID works.
This Research product is the result of merged Research products in OpenAIRE.

You have already added works in your ORCID record related to the merged Research product.
This Research product is the result of merged Research products in OpenAIRE.

You have already added works in your ORCID record related to the merged Research product.
Select content type to embed
All Research products
arrow_drop_down
<script type="text/javascript">  </script>
COPY SCRIPT
For further information contact us at helpdesk@openaire.eu
Cloning and test expression of HTT fragment clones - 2019/02/28
Research data2019IsAmongTopNSimilarDocuments
linkLink to
shareshareShare
citeCite
addClaim
Please grant OpenAIRE to access and update your ORCID works.
This Research product is the result of merged Research products in OpenAIRE.

You have already added works in your ORCID record related to the merged Research product.
This Research product is the result of merged Research products in OpenAIRE.

You have already added works in your ORCID record related to the merged Research product.
Select content type to embed
All Research products
arrow_drop_down
<script type="text/javascript">  </script>
COPY SCRIPT
For further information contact us at helpdesk@openaire.eu
more_vert
linkLink to
shareshareShare
citeCite
addClaim
Please grant OpenAIRE to access and update your ORCID works.
This Research product is the result of merged Research products in OpenAIRE.

You have already added works in your ORCID record related to the merged Research product.
This Research product is the result of merged Research products in OpenAIRE.

You have already added works in your ORCID record related to the merged Research product.
Select content type to embed
All Research products
arrow_drop_down
<script type="text/javascript">  </script>
COPY SCRIPT
For further information contact us at helpdesk@openaire.eu
Large-scale purification of Q23 and Q54 HTT from Sf9 and EXPI293F expression systems 2019/07/22
Research data2019IsAmongTopNSimilarDocuments
linkLink to
shareshareShare
citeCite
addClaim
Please grant OpenAIRE to access and update your ORCID works.
This Research product is the result of merged Research products in OpenAIRE.

You have already added works in your ORCID record related to the merged Research product.
This Research product is the result of merged Research products in OpenAIRE.

You have already added works in your ORCID record related to the merged Research product.
Select content type to embed
All Research products
arrow_drop_down
<script type="text/javascript">  </script>
COPY SCRIPT
For further information contact us at helpdesk@openaire.eu
more_vert
linkLink to
shareshareShare
citeCite
addClaim
Please grant OpenAIRE to access and update your ORCID works.
This Research product is the result of merged Research products in OpenAIRE.

You have already added works in your ORCID record related to the merged Research product.
This Research product is the result of merged Research products in OpenAIRE.

You have already added works in your ORCID record related to the merged Research product.
Select content type to embed
All Research products
arrow_drop_down
<script type="text/javascript">  </script>
COPY SCRIPT
For further information contact us at helpdesk@openaire.eu
Purification of HTT mutant samples 2019/05/17
Research data2019IsAmongTopNSimilarDocuments
linkLink to
shareshareShare
citeCite
addClaim
Please grant OpenAIRE to access and update your ORCID works.
This Research product is the result of merged Research products in OpenAIRE.

You have already added works in your ORCID record related to the merged Research product.
This Research product is the result of merged Research products in OpenAIRE.

You have already added works in your ORCID record related to the merged Research product.
Select content type to embed
All Research products
arrow_drop_down
<script type="text/javascript">  </script>
COPY SCRIPT
For further information contact us at helpdesk@openaire.eu
more_vert
linkLink to
shareshareShare
citeCite
addClaim
Please grant OpenAIRE to access and update your ORCID works.
This Research product is the result of merged Research products in OpenAIRE.

You have already added works in your ORCID record related to the merged Research product.
This Research product is the result of merged Research products in OpenAIRE.

You have already added works in your ORCID record related to the merged Research product.
Select content type to embed
All Research products
arrow_drop_down
<script type="text/javascript">  </script>
COPY SCRIPT
For further information contact us at helpdesk@openaire.eu
Expression and purification of HTT79-3144 with and without HAP40 from Sf9 Insect cells - 2019/01/28
Research data2019IsAmongTopNSimilarDocuments
linkLink to
shareshareShare
citeCite
addClaim
Please grant OpenAIRE to access and update your ORCID works.
This Research product is the result of merged Research products in OpenAIRE.

You have already added works in your ORCID record related to the merged Research product.
This Research product is the result of merged Research products in OpenAIRE.

You have already added works in your ORCID record related to the merged Research product.
Select content type to embed
All Research products
arrow_drop_down
<script type="text/javascript">  </script>
COPY SCRIPT
For further information contact us at helpdesk@openaire.eu
more_vert
linkLink to
shareshareShare
citeCite
addClaim
Please grant OpenAIRE to access and update your ORCID works.
This Research product is the result of merged Research products in OpenAIRE.

You have already added works in your ORCID record related to the merged Research product.
This Research product is the result of merged Research products in OpenAIRE.

You have already added works in your ORCID record related to the merged Research product.
Select content type to embed
All Research products
arrow_drop_down
<script type="text/javascript">  </script>
COPY SCRIPT
For further information contact us at helpdesk@openaire.eu
Purification of HTT aa. 76-3060 -HAP40 aa. 1-371 for structural analysis 2019/10/21
Research data2019IsAmongTopNSimilarDocuments
linkLink to
shareshareShare
citeCite
addClaim
Please grant OpenAIRE to access and update your ORCID works.
This Research product is the result of merged Research products in OpenAIRE.

You have already added works in your ORCID record related to the merged Research product.
This Research product is the result of merged Research products in OpenAIRE.

You have already added works in your ORCID record related to the merged Research product.
Select content type to embed
All Research products
arrow_drop_down
<script type="text/javascript">  </script>
COPY SCRIPT
For further information contact us at helpdesk@openaire.eu
more_vert
linkLink to
shareshareShare
citeCite
addClaim
Please grant OpenAIRE to access and update your ORCID works.
This Research product is the result of merged Research products in OpenAIRE.

You have already added works in your ORCID record related to the merged Research product.
This Research product is the result of merged Research products in OpenAIRE.

You have already added works in your ORCID record related to the merged Research product.
Select content type to embed
All Research products
arrow_drop_down
<script type="text/javascript">  </script>
COPY SCRIPT
For further information contact us at helpdesk@openaire.eu
Purification of HTT Q23 and Q54 from EXPI293F for mass spectrometry analysis 2019/09/30
Research data2019IsAmongTopNSimilarDocuments
linkLink to
shareshareShare
citeCite
addClaim
Please grant OpenAIRE to access and update your ORCID works.
This Research product is the result of merged Research products in OpenAIRE.

You have already added works in your ORCID record related to the merged Research product.
This Research product is the result of merged Research products in OpenAIRE.

You have already added works in your ORCID record related to the merged Research product.
Select content type to embed
All Research products
arrow_drop_down
<script type="text/javascript">  </script>
COPY SCRIPT
For further information contact us at helpdesk@openaire.eu
more_vert
linkLink to
shareshareShare
citeCite
addClaim
Please grant OpenAIRE to access and update your ORCID works.
This Research product is the result of merged Research products in OpenAIRE.

You have already added works in your ORCID record related to the merged Research product.
This Research product is the result of merged Research products in OpenAIRE.

You have already added works in your ORCID record related to the merged Research product.
Select content type to embed
All Research products
arrow_drop_down
<script type="text/javascript">  </script>
COPY SCRIPT
For further information contact us at helpdesk@openaire.eu

chevron_left
1
2
chevron_right

Impact byBIP!

	citations This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).	0
	popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.	Average
	influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).	Average
	impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.	Average