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Analysis of dephosphorylated HTT samples by cryoEM analysis 2019/05/01

Research datakeyboard_double_arrow_right Dataset 01 May 2019Publisher:Zenodo

Authors: Harding, Rachel; Deme, Justin; Lea, Susan; Arrowsmith, Cheryl; Edwards, Aled;

doi: 10.5281/zenodo.3234166 , 10.5281/zenodo.3234167

Analysis of dephosphorylated HTT samples by cryoEM analysis 2019/05/01

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Abstract

Dr. Harding is the recipient of the Huntington's Disease Society of America Berman Topper Career Development Fellowship which funds and supports this research, in addition to generous funding from the CHDI Foundation and the Huntington Society of Canada. The SGC is a registered charity (number 1097737) that receives funds from AbbVie, Bayer Pharma AG, Boehringer Ingelheim, Canada Foundation for Innovation, Eshelman Institute for Innovation, Genome Canada through Ontario Genomics Institute [OGI-055], Innovative Medicines Initiative (EU/EFPIA) [ULTRA-DD grant no. 115766], Janssen, Merck KGaA, Darmstadt, Germany, MSD, Novartis Pharma AG, Ontario Ministry of Research, Innovation and Science (MRIS), Pfizer, São Paulo Research Foundation-FAPESP, Takeda, and Wellcome.

Project: High resolution structural analysis of purified HTT samples Experiment: Analysis of dephosphorylated HTT samples by cryoEM analysis Date completed: 2019/05/01 Rationale: We now have a good understanding of the global structure of HTT when in complex with HAP40 and our MALS and SAXS data suggest that apo HTT is likely self-associating and heterogenous in nature, despite high levels of purity. Dephosphorylating HTT from Sf9 cell production could alter its global structure sufficiently to allow high resolution structure determination by cryoEM.

Related Organizations

University of Oxford
United Kingdom
University of Toronto
Canada

Keywords

HD, Huntington's disease, Huntingtin, HTT, Phosphorylation, CryoEM

15 Research products, page 1 of 2

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Analysis of dephosphorylated HTT samples for cryoEM analysis and further sample preparation 2019/04/15
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Large-scale purification of Q23 HTT from Sf9 2019/09/30
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Purification of HTT mutant samples 2019/05/17
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Coexpression and purification of HTT domains with HAP40 - 2018/12/03
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Cloning and test expression of HTT fragment clones - 2019/02/28
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Large-scale purification of Q23 and Q54 HTT from Sf9 and EXPI293F expression systems 2019/07/22
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Multi-angle light scattering (MALS) analysis of HTT1-3144 Q54 HAP401-371 samples purified from Sf9 insect cells - 2018/12/03
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Expression and purification of HTT79-3144 with and without HAP40 from Sf9 Insect cells - 2019/01/28
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Purification of HTT aa. 76-3060 -HAP40 aa. 1-371 for structural analysis 2019/10/21
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