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Purification of HTT Q23 and Q54 from EXPI293F for mass spectrometry analysis 2019/09/30

Research datakeyboard_double_arrow_right Dataset 30 Sep 2019Publisher:Zenodo

Authors: Harding, Rachel; Hutchinson, Ashley; Seitova, Alma; Arrowsmith, Cheryl; Edwards, Aled;

doi: 10.5281/zenodo.3555361 , 10.5281/zenodo.3555360

Purification of HTT Q23 and Q54 from EXPI293F for mass spectrometry analysis 2019/09/30

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Abstract

Dr. Harding is the recipient of the Huntington's Disease Society of America Berman Topper Career Development Fellowship which funds and supports this research, in addition to generous funding from the CHDI Foundation and the Huntington Society of Canada. The SGC is a registered charity (number 1097737) that receives funds from AbbVie, Bayer Pharma AG, Boehringer Ingelheim, Canada Foundation for Innovation, Eshelman Institute for Innovation, Genome Canada through Ontario Genomics Institute [OGI-055], Innovative Medicines Initiative (EU/EFPIA) [ULTRA-DD grant no. 115766], Janssen, Merck KGaA, Darmstadt, Germany, MSD, Novartis Pharma AG, Ontario Ministry of Research, Innovation and Science (MRIS), Pfizer, São Paulo Research Foundation-FAPESP, Takeda, and Wellcome.

Project: Biophysical investigation of purified HTT protein samples Experiment: Purification of Q23 and Q54 HTT from EXPI293F Date completed: 2019/09/30 Rationale: Purify HTT Q23 and Q54 for mass spectrometry analysis

Related Organizations

University of Toronto
Canada

Keywords

huntington's disease, huntingtin, protein purification, mass spectrometry

15 Research products, page 1 of 2

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Analysis of dephosphorylated HTT samples for cryoEM analysis and further sample preparation 2019/04/15
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Coexpression and purification of HTT domains with HAP40 - 2018/12/03
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Large-scale purification of Q23 HTT from Sf9 2019/09/30
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Cloning and test expression of HTT fragment clones - 2019/02/28
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Large-scale purification of Q23 and Q54 HTT-HAP40 from Sf9 expression system 2019/07/29
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Analysis of dephosphorylated HTT samples by cryoEM analysis 2019/05/01
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Multi-angle light scattering (MALS) analysis of HTT1-3144 Q54 HAP401-371 samples purified from Sf9 insect cells - 2018/12/03
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Purification of HTT mutant samples 2019/05/17
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A time-course analysis using Differential Static Light Scattering (DSLS) of purified HTT1-3144 Q23 - 2019/01/28
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