Supplementary Movie S2. Serial blockface fluorescence imaging in SBF-SEM with miniLM.
Brama, Elisabeth; J. Peddie, Christopher; Wilkes, Gary; Gu, Yan; M. Collinson, Lucy; L. Jones, Martin;
- Publisher: Figshare
- Subject: GFP | ultraLM | In-resin fluorescence | Cell Biology | miniLM | Correlative | Serial block face SEM | Ultramicrotome | CLEM | Integrated | Smart tracking | Locator tool
<p>Slices acquired at 100 nm thickness with the miniLM operating inside the scanning electron microscope. The outer circle shows the edge of the fibre bundle and the effect of the discrete fibre cores is seen as a fixed-pattern pixellation across the surface.</... View more