Electron Microscopy of Living Cells During in Situ Fluorescence Microscopy

Audiovisual UNKNOWN
Liv, Nalan; S. B. van Oosten Slingeland, Daan; Baudoin, Jean-Pierre; Kruit, Pieter; Piston, David W.; Hoogenboom, Jacob P.;
(2016)
  • Publisher: Figshare
  • Related identifiers: doi: 10.1021/acsnano.5b03970.s002, doi: 10.1021/acsnano.5b03970.s003, doi: 10.1021/acsnano.5b03970.s004, doi: 10.1021/acsnano.5b03970.s005
  • Subject: Biotechnology | visualization | extension | fluorescence imaging | Plant Biology | approach | microscope | Situ Fluorescence MicroscopyWe | cells encapsulated | Living Cells | bionanoreactor | electron Microscopy | SEM | Biophysics | scanning electron microscopy | Inorganic Chemistry | uptake | Microbiology | EM resolution | Medicine | Cell Biology | bioconjugated quantum dots | Biochemistry | internalization | Cancer | location | nanoimaging | region | snapshot
    • FOR: 29999 Physical Sciences not elsewhere classified | 39999 Chemical Sciences not elsewhere classified

We present an approach toward dynamic nanoimaging: live fluorescence of cells encapsulated in a bionanoreactor is complemented with <i>in situ</i> scanning electron microscopy (SEM) on an integrated microscope. This allows us to take SEM snapshots on-demand, th... View more
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