Hydrophobic Interaction Chromatography for Bottom-Up Proteomics Analysis of Single Proteins and Protein Complexes

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Rackiewicz, Michal; Große-Hovest, Ludger; Alpert, Andrew J.; Zarei, Mostafa; Dengjel, Jörn;
(2017)
  • Publisher: Figshare
  • Related identifiers: doi: 10.1021/acs.jproteome.7b00015.s003, doi: 10.1021/acs.jproteome.7b00015.s002, doi: 10.1021/acs.jproteome.7b00015.s004
  • Subject: peptide mapping | Biotechnology | HIC | RPC | study protein complexes | MS | Bottom-Up Proteomics Analysis | Medicine | Protein Complexes Hydrophobic interaction chromatography | bottom-up proteomics experiments | study post-translational modifications | Hydrophobic Interaction Chromatography | Biochemistry | fractionate antibody species | LC | Biophysics | fractionate proteins | Single Proteins
    • FOR: 29999 Physical Sciences not elsewhere classified | 69999 Biological Sciences not elsewhere classified | 39999 Chemical Sciences not elsewhere classified
    mesheuropmc: endocrine system

Hydrophobic interaction chromatography (HIC) is a robust standard analytical method to purify proteins while preserving their biological activity. It is widely used to study post-translational modifications of proteins and drug–protein interactions. In the c... View more
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